Genetic variations and dog breed identification using inter-simple sequence repeat markers coupled with high resolution melting analysis

PeerJ
Wannapimol KriangwanichChatchote Thitaram

Abstract

The identification of differing physical characteristics of dogs is an uncomplicated and straightforward way to categorize dog breeds. However, many dog owners and veterinarians still struggle to distinguish between pure breed and mixed variations in certain breeds of dogs. Presently, the absence of the tools and methods needed to confirm a pure breed dog is a significant problem since the only method available to validate pure or mongrel breeds is the official pedigree system. Inter-simple sequence repeat markers have been successfully used to assess genetic variations and differentiations. Notably, inter-simple sequence repeat markers coupled with high resolution melting analysis were effectively used for the breed identification of 43 breeds of dogs (total 463 dogs). The 10 primers chosen for analysis resulted in a range of 31-78.6% of breed discrimination when using one primer, while a combination of two primers was able to successfully discriminate between all of the 43 dog breeds (100%). Shannon's index information (I = 2.586 ± 0.034) and expected heterozygosity (H e  = 0.908 ± 0.003) indicated a high level of genetic diversity among breeds. The fixation index (F st ) revealed a value of 10.4%, demonstrating that there ...Continue Reading

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Citations

Jun 4, 2021·Scientific Reports·Anocha PoommouangKorakot Nganvongpanit

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Datasets Mentioned

BETA
UBC844

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

STURCTUE
Eco
GenAlEx
STRUCTURE
Excel
DARwin

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