Genetic variations in regions of bovine and bovine-like enteroviral 5'UTR from cattle, Indian bison and goat feces

Virology Journal
Nathamon KosoltanapiwatSasipan Pearsiriwuttipong

Abstract

Bovine enteroviruses (BEV) are members of the genus Enterovirus in the family Picornaviridae. They are predominantly isolated from cattle feces, but also are detected in feces of other animals, including goats and deer. These viruses are found in apparently healthy animals, as well as in animals with clinical signs and several studies reported recently suggest a potential role of BEV in causing disease in animals. In this study, we surveyed the presence of BEV in domestic and wild animals in Thailand, and assessed their genetic variability. Viral RNA was extracted from fecal samples of cattle, domestic goats, Indian bison (gaurs), and deer. The 5' untranslated region (5'UTR) was amplified by nested reverse transcription-polymerase chain reaction (RT-PCR) with primers specific to BEV 5'UTR. PCR products were sequenced and analyzed phylogenetically using the neighbor-joining algorithm to observe genetic variations in regions of the bovine and bovine-like enteroviral 5'UTR found in this study. BEV and BEV-like sequences were detected in the fecal samples of cattle (40/60, 67 %), gaurs (3/30, 10 %), and goats (11/46, 24 %). Phylogenetic analyses of the partial 5'UTR sequences indicated that different BEV variants (both EV-E and EV-...Continue Reading

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Citations

Apr 5, 2017·PloS One·Mingyue WangXinping Wang
Dec 16, 2018·Applied and Environmental Microbiology·Nicharee IncomeIrwin Fernandez Chavez

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Datasets Mentioned

BETA
DQ092794.1
KT992123
KT992107
AF123432.1
KF748290.1
DQ092769.1
KM887134.1
JQ277724.1
AY462106.1
AB857843.2

Methods Mentioned

BETA
PCR
nucleic acid amplifications

Software Mentioned

Geographics
BLAST
ClustalW
ESRI® ArcGIS
Aerogrid
GeoEye
MEGA
Swisstopo
CAP ( Contig Assembly Program )
Getmapping

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