Genetic visualization of the secondary olfactory pathway in Tbx21 transgenic mice.

Neural Systems & Circuits
Sachiko MitsuiYoshihiro Yoshihara

Abstract

Mitral and tufted cells are the projection neurons in the olfactory bulb, conveying odour information to various regions of the olfactory cortex. In spite of their functional importance, there are few molecular and genetic tools that can be used for selective labelling or manipulation of mitral and tufted cells. Tbx21 was first identified as a T-box family transcription factor regulating the differentiation and function of T lymphocytes. In the brain, Tbx21 is specifically expressed in mitral and tufted cells of the olfactory bulb. In this study, we performed a promoter/enhancer analysis of mouse Tbx21 gene by comparing nucleotide sequence similarity of Tbx21 genes among several mammalian species and generating transgenic mouse lines with various lengths of 5' upstream region fused to a fluorescent reporter gapVenus. We identified the cis-regulatory enhancer element (~300 nucleotides) at ~ 3.0 kb upstream of the transcription start site of Tbx21 gene, which is both necessary and sufficient for transgene expression in mitral and tufted cells. In contrast, the 2.6-kb 5'-flanking region of mouse Tbx21 gene induced transgene expression with variable patterns in restricted populations of neurons predominantly located along the olfac...Continue Reading

References

Oct 1, 1995·Progress in Neurobiology·I Naruse, H Keino
Nov 15, 1996·Cell·P MombaertsR Axel
Mar 5, 1999·Brain Research. Developmental Brain Research·K Hagino-YamagishiK Yazaki
Jul 17, 1999·Brain Research. Developmental Brain Research·N KimuraT Taga
Jan 10, 2001·Development, Growth & Differentiation·M Tada, J C Smith
Jul 20, 2002·Mechanisms of Development·Andrea FaedoAlessandro Bulfone
Aug 15, 2003·Journal of Neurophysiology·Kevin R Neville, Lewis B Haberly
Apr 6, 2004·The Journal of Comparative Neurology·Curtis William Balmer, Anthony-Samuel LaMantia
Jan 29, 2005·Development·Sei-ichi YoshiharaYoshihiro Yoshihara
Feb 4, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sachiko MitsuiYoshihiro Yoshihara
Apr 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhiying LiVenkatesh N Murthy
Aug 18, 2005·Genesis : the Journal of Genetics and Development·Yumiko NagaiMineto Yokoi
Nov 16, 2005·Annual Review of Genetics·L A NaicheVirginia E Papaioannou
Feb 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hitomi MatsunoYoshihiro Yoshihara
Jul 4, 2006·Brain Research·Friedrich LaubFrancesco Ramirez
Aug 24, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ikue Yoshida, Kensaku Mori
Nov 9, 2007·Nature·Ko KobayakawaHitoshi Sakano
Mar 28, 2008·Neuron·Tomomi Kaneko-GotoYoshihiro Yoshihara
Apr 17, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nobuhiko MiyasakaYoshihiro Yoshihara
Jul 8, 2009·Molecular and Cellular Neurosciences·Weiguo FengTrevor Williams

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Citations

May 8, 2013·Trends in Neurosciences·John M Bekkers, Norimitsu Suzuki
Dec 24, 2013·Trends in Neurosciences·Arie S MobleyCharles A Greer
Apr 10, 2014·Nature Communications·Nobuhiko MiyasakaYoshihiro Yoshihara
Nov 14, 2014·The European Journal of Neuroscience·Fumiaki Imamura, Charles A Greer
Aug 2, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Eva Díaz-GuerraCarlos Vicario-Abejón
Mar 20, 2016·Cellular and Molecular Life Sciences : CMLS·Dhananjay Huilgol, Shubha Tole
Nov 22, 2011·Neuron·Donald A Wilson, Regina M Sullivan
Feb 28, 2014·Adipocyte·Emilie StolarczykJane K Howard
Aug 19, 2018·The European Journal of Neuroscience·Enrico Bagnoli, Una FitzGerald
Mar 14, 2019·The Journal of Comparative Neurology·Uyen P Nguyen, Fumiaki Imamura
May 13, 2020·Nature Neuroscience·Kimiya NarikiyoYoshihiro Yoshihara
Jul 31, 2019·Nature Communications·Gary LiuBenjamin R Arenkiel

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Methods Mentioned

BETA
transgenic
Fluorescence
PCR

Software Mentioned

IDL
LabVIEW
Adobe Photoshop
MatInspector
VISTA

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