Genetically abnormal circulating cells in lung cancer patients: an antigen-independent fluorescence in situ hybridization-based case-control study.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Ruth L KatzRanda El-Zein

Abstract

We performed a study to determine if a fluorescence in situ hybridization (FISH)-based assay using isolated peripheral blood mononuclear cells (PBMCs) with DNA probes targeting specific sites on chromosomes known to have abnormalities in non-small cell lung cancer (NSCLC) cases could detect circulating genetically abnormal cells (CACs). We evaluated 59 NSCLC cases with stage I through IV disease and 24 controls. PBMCs and matched tumors were hybridized with 2 two-color [3p22.1/CEP3 and 10q22.3 (SP-A)/CEP10) and 2 four-color [CEP3, CEP7, CEP17, and 9p21.3 (URO); and EGFR, c-MYC, 6p11-q11, and 5p15.2 (LAV)] FISH probes. Percentages of cytogenetically abnormal cells (CACs) in peripheral blood and in matched tumor specimens were quantified by using an automated fluorescent scanner. Numbers of CACs were calculated based on the percentage of CACs (defined as PBMCs with genetic abnormalities) per milliliter of blood and expressed per microliter of blood. Patients with NSCLC had significantly higher numbers of CACs than controls. Mean number of CACs ranged from 7.23 +/- 1.32/microL for deletions of 10q22.3/CEP10 to 45.52 +/- 7.49/microL for deletions of 3p22.1/CEP3. Numbers of CACs with deletions of 3p22.1, 10q22.3, and 9p21.3, and gai...Continue Reading

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Citations

Mar 6, 2013·Nature Reviews. Clinical Oncology·Howard I ScherGlenn Heller
Apr 12, 2013·Science Translational Medicine·Lixin ZhangDario Marchetti
Aug 9, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lingmin HuHongbing Shen
Aug 2, 2013·Molecular Cancer·Cyndia CharfiEric Rassart
Apr 17, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Jonathan W UhrHarold R Garner
May 1, 2011·Experimental and Therapeutic Medicine·Chuanli RenHongbing Shen
Apr 20, 2018·Diagnostics·Peter Ping Lin
Apr 25, 2021·Journal of Cancer Research and Clinical Oncology·Xiaochang QiuDianjie Lin

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