Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo.

Neuroscience Research
Masatoshi Inoue

Abstract

Over the past two decades, genetically encoded calcium indicators (GECIs) have been used extensively to report intracellular calcium (Ca2+) dynamics in order to readout neuronal and network activity in living tissue. Single wavelength GECIs, such as GCaMP, have been widely adapted due to advances in dynamic range, sensitivity, and kinetics. Additionally, recent efforts in protein engineering have expanded the GECI color palette to enable direct optical interrogation of more complex circuit dynamics. Here, I discuss the engineering, application, and future directions of the most recently developed GECIs for in vivo neuroscience research.

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Citations

Oct 31, 2020·Frontiers in Neuroscience·Ricardo Augusto de Melo ReisFernando Garcia de Mello
Mar 13, 2021·Journal of Neuroscience Methods·Ronit HeinrichShai Berlin
Jun 18, 2021·ELife·David J Marcus, Michael R Bruchas
Jun 29, 2021·Frontiers in Cellular Neuroscience·Christian LohrChristine E Gee
Aug 28, 2021·Cells·Nelly RedolfiDiana Pendin
Sep 18, 2021·Cold Spring Harbor Protocols·Hollis T Cline

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