Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway.

Proceedings of the National Academy of Sciences of the United States of America
Daisy W LeungMichael K Rosen

Abstract

General methods to engineer genetically encoded, reversible, light-mediated control over protein function would be useful in many areas of biomedical research and technology. We describe a system that yields such photo-control over actin assembly. We fused the Rho family GTPase Cdc42 in its GDP-bound form to the photosensory domain of phytochrome B (PhyB) and fused the Cdc42 effector, the Wiskott-Aldrich Syndrome Protein (WASP), to the light-dependent PhyB-binding domain of phytochrome interacting factor 3 (Pif3). Upon red light illumination, the fusion proteins bind each other, activating WASP, and consequently stimulating actin assembly by the WASP target, the Arp2/3 complex. Binding and WASP activation are reversed by far-red illumination. Our approach, in which the biochemical specificity of the nucleotide switch in Cdc42 is overridden by the light-dependent PhyB-Pif3 interaction, should be generally applicable to other GTPase-effector pairs.

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·L Li, J C Lagarias
Jan 1, 1993·Annual Review of Physiology·S R Adams, R Y Tsien
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SpoernerA Wittinghofer
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·G A Gambetta, J C Lagarias
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Peter H Quail
Sep 10, 2002·Nature Biotechnology·Sae Shimizu-SatoPeter H Quail
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark E HatleyRama Ranganathan
Apr 16, 2004·Current Biology : CB·Mike LorenzJohn Condeelis
Dec 1, 2004·Annual Review of Genetics·Meng ChenChristian Fankhauser
Feb 3, 2005·The Journal of Cell Biology·Hideki YamaguchiJohn Condeelis
Apr 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Daisy W Leung, Michael K Rosen
Aug 9, 2005·Trends in Cell Biology·Deborah M RothmanBarbara Imperiali
Jan 24, 2006·Chemistry & Biology·Laura A Banaszynski, Thomas J Wandless
Feb 14, 2006·Methods in Enzymology·Daisy W LeungMichael K Rosen
Apr 28, 2006·Chembiochem : a European Journal of Chemical Biology·Christian Renner, Luis Moroder
May 4, 2006·Annual Review of Plant Biology·Nathan C RockwellJ Clark Lagarias
Sep 23, 2006·Nature Methods·Feng ZhangKarl Deisseroth
Aug 21, 2007·Applied Microbiology and Biotechnology·Annette Müller-Taubenberger, Kurt I Anderson

❮ Previous
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Citations

Feb 19, 2013·Pflügers Archiv : European journal of physiology·Taofei Yin, Yi I Wu
Sep 29, 2011·Journal of Biomolecular NMR·Pilong LiMichael K Rosen
Oct 5, 2011·ACS Chemical Biology·Brenda N Goguen, Barbara Imperiali
Nov 3, 2010·Nature Methods·Matthew J KennedyChandra L Tucker
Sep 13, 2011·Nature Methods·Jared E ToettcherOrion D Weiner
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kyoung Hu LeeWon Do Heo
Mar 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gregory T KappTanja Kortemme
Mar 1, 2012·The Plant Cell·David W Ehrhardt, Wolf B Frommer
Sep 19, 2009·Journal of Biological Engineering·Oxana SorokinaAndrew J Millar
Oct 2, 2013·Planta·Arash Kianianmomeni, Armin Hallmann
Aug 9, 2011·Annual Review of Cell and Developmental Biology·Gero Miesenböck
Mar 20, 2013·Annual Review of Plant Biology·Sidney L Shaw, David W Ehrhardt
Mar 3, 2010·Annual Review of Plant Biology·Andreas MöglichKeith Moffat
Jan 31, 2013·Chemical Society Reviews·Kiryl D PiatkevichVladislav V Verkhusha
Feb 14, 2012·Current Opinion in Biotechnology·Ellen C O'Shaughnessy, Casim A Sarkar
Jun 15, 2011·Current Opinion in Cell Biology·Niña C Hartman, Jay T Groves
Sep 21, 2010·Current Opinion in Neurobiology·Anshul Rana, Ricardo E Dolmetsch
Mar 18, 2011·British Journal of Pharmacology·Michael Patterson, Ryohei Yasuda
Nov 20, 2012·Biology of the Cell·Gopal P PathakChandra L Tucker
Jun 8, 2011·Biotechnology Journal·Daniel CamsundAlfonso Jaramillo
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Joanne Chory
Oct 8, 2013·Biochemical and Biophysical Research Communications·Zhengcai MaYi Yang
Sep 22, 2009·Biochimica Et Biophysica Acta·Melanie A Priestman, David S Lawrence
Mar 21, 2012·Molecular Plant·John M ChristieAndrew J Roe
Sep 13, 2014·Current Opinion in Cell Biology·Matthew Weitzman, Klaus M Hahn
Apr 7, 2010·Journal of Molecular Biology·Stacy-Anne MorganG Andrew Woolley
Nov 8, 2011·Nature Biotechnology·Andreas Milias-ArgeitisJohn Lygeros
Jul 4, 2016·Current Opinion in Chemical Biology·Barbara Di Ventura, Brian Kuhlman
Jun 14, 2016·Biochemistry·Nathan C RockwellJ Clark Lagarias
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Doug Tischer, Orion D Weiner
Jul 3, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·P R O'Neill, N Gautam
Mar 27, 2018·Chembiochem : a European Journal of Chemical Biology·Yoshibumi Ueda, Moritoshi Sato
Oct 31, 2009·Chembiochem : a European Journal of Chemical Biology·Katryn R Harwood, Stephen C Miller
Jan 12, 2010·Chembiochem : a European Journal of Chemical Biology·Wesleigh E Georgianna, Alexander Deiters
Sep 14, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Andreas Möglich, Keith Moffat
Jun 18, 2014·Nature Chemical Biology·Arnaud GautierSophie Vriz
Jun 13, 2012·Chemistry : a European Journal·Jeung-Hoi Ha, Stewart N Loh

❮ Previous
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