Genetically engineered drug rhCNB induces apoptosis and cell cycle arrest in both gastric cancer cells and hepatoma cells

Drug Design, Development and Therapy
Yanzi GuoJian Fu

Abstract

Calcineurin B (CNB) is a regulatory subunit of calcineurin, and it has antitumor activity. In this study, we aimed to investigate the effect of recombinant human calcineurin B (rhCNB) on the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. Cell viability and cell proliferation were detected by MTT and BrdU assay. Flow cytometry, Western blot and immunohistochemistry were performed to determine rhCNB-induced apoptosis and cell cycle arrest. The antitumor activities of rhCNB were observed in mice tumor models. We demonstrated that rhCNB inhibits the proliferation of gastric cancer cells and hepatoma cells both in vitro and in vivo. We showed that the inhibition of cell proliferation by rhCNB is associated with apoptosis and cell cycle arrest in both tumor cell lines. Furthermore, we indicated that rhCNB promotes p53 protein expression, a potent proapoptotic factor. Meanwhile, we also exhibited that rhCNB decreases the expression of both cyclin B1 and CDK1 proteins, two proteins associated with G2/M arrest. Together, these findings suggest that rhCNB markedly inhibits tumor growth and provides guidance for its drug development.

Citations

May 22, 2019·Journal of Cellular Biochemistry·Wenshuang YuanJianwen Liu
Apr 23, 2020·International Journal of Oncology·An-Gui LiuXin-Gan Qin

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Assay
protein assay
electrophoresis
bioluminescence imaging
flow cytometry

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis