Genetically engineered poly-gamma-glutamate producer from Bacillus subtilis ISW1214

Bioscience, Biotechnology, and Biochemistry
Makoto AshiuchiHaruo Misono

Abstract

The pgsBCA-gene disruptant from Bacillus subtilis ISW1214, i.e., MA41, does not produce poly-gamma-glutamate (PGA). We newly constructed an MA41 recombinant bearing the plasmid-borne PGA synthetic system, in which PGA production was strictly controlled by the use of xylose. Unlike the parent strain, ISW1214, the genetically engineered strain produced abundant PGA in both L-glutamate-rich and D-glutamate-rich media.

References

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Oct 19, 2001·European Journal of Biochemistry·Makoto AshiuchiHaruo Misono
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Citations

Dec 17, 2014·Microbial Cell Factories·Kiyotaka Y HaraAkihiko Kondo
Mar 8, 2011·Bioresource Technology·Ishwar Bajaj, Rekha Singhal
Aug 28, 2007·Biochemical and Biophysical Research Communications·Makoto AshiuchiHaruo Misono
Sep 3, 2010·Biotechnology and Bioengineering·Daisuke YamashiroMakoto Ashiuchi
Apr 14, 2016·BioMed Research International·Biaosheng LinMaochun Luo
Oct 8, 2014·Microbiology·Adetoro OgunleyeIza Radecka
Dec 8, 2017·International Journal of Molecular Sciences·Yi-Huang HsuehTzong-Yi Lee

Related Concepts

Gamma-polyglutamate
Natto Bacteria
Genetic Engineering
Episomes
Polyglutamate
Molecular Stereochemistry
Xylose
Glutamic Acid, (D)-Isomer

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