PMID: 8607687Apr 1, 1996Paper

Genetically engineered serine protease inhibitor for hemostasis after cardiac operations

The Annals of Thoracic Surgery
S K OhriK M Taylor

Abstract

The serine protease inhibitor aprotinin has been widely reported for its beneficial action in limiting blood loss after cardiopulmonary bypass (CPB). A potent human serine protease inhibitor known as protease nexin II or amyloid precursor protein has been recently isolated. A recombinant protein known as recombinant Kunitz protease inhibitor (rKPI; Scios Nova, Mountain View, CA) with sequence homology to the protease nexin II-amyloid precursor protein molecule has been manufactured. Recombinant Kunitz protease inhibitor was assessed in an ovine model of CPB as a hemostatic agent after CPB. Sheep (n = 22) underwent CPB for 90 minutes. Two thoracic drains were sited and drain losses collected for a period of 3 hours after CPB. Wounds were subjectively assessed before closure for "dryness" using a visual analogue scale. Sheep were randomized to control (n = 8), aprotinin (n = 8), and rKPI (n = 6) groups. Control animals had a drain loss of 409.4 +/- 39.4 mL/3 h, compared with 131.3 +/- 20.3 mL/3 h for the aprotinin group and 163.7 +/- 34.3 mL/3 h for the rKPI group (p = 0.16). Hemoglobin loss was 11.6 +/- 3.6, 6.02 +/- 2.1, and 4.6 +/- 1.2 g/3 h for the control, rKPI, and aprotinin groups respectively (p = 0.25). The subjective an...Continue Reading

References

May 29, 1992·Science·J Marx
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C L MastersK Beyreuther
May 29, 1986·The New England Journal of Medicine·L A Harker

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Citations

Sep 2, 1998·The Annals of Thoracic Surgery·I NagahiroG A Patterson
Mar 16, 2001·Expert Opinion on Pharmacotherapy·P P PunjabiK M Taylor
Mar 1, 1997·European Journal of Anaesthesiology. Supplement·B J Hunt

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