Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection

Advanced Healthcare Materials
Yanyan HuaiChuanbin Mao

Abstract

Candida albicans (CA) is a kind of fungus that can cause high morbidity and mortality in immunocompromised patients. However, preventing CA infection in these patients is still a daunting challenge. Herein, inspired from the fact that immunization with secreted aspartyl proteinases 2 (Sap2) can prevent the infection, it is proposed to use filamentous phage, a human-safe virus nanofiber specifically infecting bacteria (≈900 nm long and 7 nm wide), to display an epitope peptide of Sap2 (EPS, with a sequence of Val-Lys-Tyr-Thr-Ser) on its side wall and thus serve as a vaccine for preventing CA infection. The engineered virus nanofibers and recombinant Sap2 (rSap2) are then separately used to immunize mice. The humoral and cellular immune responses in the immunized mice are evaluated. Surprisingly, the virus nanofibers significantly induce mice to produce strong immune response as rSap2 and generate antibodies that can bind Sap2 and CA to inhibit the CA infection. Consequently, immunization with the virus nanofibers in mice dramatically increases the survival rate of CA-infected mice. All these results, along with the fact that the virus nanofibers can be mass-produced by infecting bacteria cost-effectively, suggest that virus nano...Continue Reading

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Citations

Jan 8, 2017·Annual Review of Pharmacology and Toxicology·Michael R Yeaman, John P Hennessey
Aug 6, 2016·Angewandte Chemie·Kegan S SunderlandChuanbin Mao
Jan 22, 2019·Angewandte Chemie·Mingying YangChuanbin Mao
Feb 14, 2017·Nano Convergence·Julius A Edson, Young Jik Kwon
Jan 29, 2020·Bioengineering & Translational Medicine·Krystina L Hess, Christopher M Jewell
Mar 10, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hong XuChuanbin Mao
Dec 15, 2020·Current Opinion in Biotechnology·Christiaan R de VriesPaul L Bollyky
Jul 10, 2018·Advanced Drug Delivery Reviews·Pan TaoVenigalla B Rao
Jul 21, 2017·Chemical Reviews·Mingying YangChuanbin Mao

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