Genetically fused charged peptides induce rapid crystallization of proteins

Chemical Communications : Chem Comm
K MinamihataT Nagamune

Abstract

We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

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Citations

Jan 21, 2021·Journal of the American Chemical Society·Kiefer O RambergPeter B Crowley
Aug 26, 2021·Chemistry : a European Journal·Kiefer O RambergPeter B Crowley

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