Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods

Toxicology Letters
L H ThompsonJ S Felton

Abstract

We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.

References

Apr 1, 1992·Carcinogenesis·M H BuonaratiJ S Felton
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Citations

Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·L WarzechaC Dobosz
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E MuckelH R Glatt
Jun 29, 2010·Journal of Applied Toxicology : JAT·Ana Isabel Haza, Paloma Morales
Jul 3, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Marko PezdircMetka Filipič
Dec 7, 2002·Nutrition and Cancer·Beverly A MontgomeryAnane Aidoo
Oct 20, 2009·Nutrition and Cancer·Wei Zheng, Sang-Ah Lee

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