Genetically modified human keratinocytes overexpressing PDGF-A enhance the performance of a composite skin graft

Human Gene Therapy
S A EmingJ R Morgan

Abstract

Skin loss due to burns and ulcers is a major medical problem. Bioengineered skin substitutes that use cultured keratinocytes as an epidermal layer with or without analogues of the dermis are one strategy for skin repair. However, none can achieve definitive wound closure, function, or cosmesis comparable to split-thickness autografts. Moreover, autograft donor sites, which require time to heal, may be limited or have attendant problems such as infection or functional/cosmetic deficiencies. To determine if the performance of composite skin grafts of keratinocytes on a dermal analogue could be enhanced, human keratinocytes were genetically modified to overexpress platelet-derived growth factor A chain (PDGF-A). Composite grafts of modified keratinocytes seeded onto acellular dermis, prepared from cryopreserved cadaver skin, secreted PDGF-AA protein in vitro [90 ng/graft (1.5 x 1.5 cm)/24 hr]. To test their performance in a wound healing model, composite grafts were transplanted to full-thickness excisional wounds on the back of athymic mice. PDGF-A grafts formed a stratified differentiated epidermis similar to control grafts. The acellular dermis was repopulated with host fibrovascular cells and by day 7, the PDGF-A grafts had si...Continue Reading

References

Mar 1, 1991·Journal of Cellular Biochemistry·N L ParenteauE Bell
Jan 23, 1987·Science·J Folkman, M Klagsbrun
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
Aug 1, 1989·Journal of the American Academy of Dermatology·T J PhillipsB A Gilchrest
Nov 1, 1989·The Journal of Dermatologic Surgery and Oncology·T J Phillips, B A Gilchrest
Sep 1, 1988·Annals of Surgery·D HeimbachG Warden
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O Danos, R C Mulligan
May 1, 1984·The Journal of Clinical Endocrinology and Metabolism·D R Clemmons
Mar 1, 1996·The Journal of Investigative Dermatology·E S FenjvesL B Taichman
May 20, 1997·Human Gene Therapy·K A Choate, P A Khavari
Mar 1, 1995·Physical Review. D·G Lazarides, C Panagiotakopoulos
Apr 5, 1994·Biotechnology and Bioengineering·L M WilkinsN L Parenteau

❮ Previous
Next ❯

Citations

Jun 23, 2006·Der Unfallchirurg·A OberholzerW Ertel
Feb 6, 2004·Blood Cells, Molecules & Diseases·Vincent Falanga
Feb 5, 2004·Journal of the American College of Surgeons·Robert L Sheridan, Ronald G Tompkins
Jul 16, 2003·British Journal of Plastic Surgery·Tan QianLin Zihao
Jan 10, 1998·British Journal of Plastic Surgery·S A EmingA Berger
Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·Kenneth R Cutroneo, Jen-Fu Chiu
Jun 21, 2002·Journal of Internal Medicine·P A KhavariA Vahlquist
Apr 12, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·D M SuppS T Boyce
Jun 3, 1999·Proceedings of the Association of American Physicians·J C Vogel
Jun 3, 1999·Proceedings of the Association of American Physicians·G G KruegerM J Petersen
Nov 21, 2000·Human Gene Therapy·S Ghazizadeh, L B Taichman
Sep 15, 2004·Tissue Engineering·David J GeerStelios T Andreadis
Mar 12, 2009·The Journal of Trauma·Michael J SchurrB Lynn Allen-Hoffmann
Oct 1, 2013·BioMed Research International·Bommie F SeoSung-No Jung
Jan 5, 2002·Expert Opinion on Biological Therapy·Jonathan Mansbridge
Nov 4, 2000·Expert Opinion on Investigational Drugs·W Pfützner, J C Vogel
Jun 4, 2004·Expert Opinion on Biological Therapy·Stelios T Andreadis
Sep 1, 2004·Expert Opinion on Biological Therapy·Sabine A EmingJeffrey M Davidson
Jun 7, 2006·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy
Nov 24, 2005·Journal of Tissue Viability·Nicola C PetrieElof Eriksson
Jan 11, 2008·The Journal of Investigative Dermatology·María J EscámezMarcela Del Río
Oct 16, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Samina BashirFarah Khan
Dec 22, 2004·The Journal of Investigative Dermatology·María José EscámezMarcela Del Río
Feb 14, 2002·The Journal of Investigative Dermatology·Danièle NoëlMarc Piechaczyk
Sep 1, 1999·The Journal of Investigative Dermatology·K W LiechtyT M Crombleholme
Jul 8, 2008·Burns : Journal of the International Society for Burn Injuries·Ludwik K BranskiMarc G Jeschke
Jan 27, 2006·The American Journal of Pathology·Detlev RothSabine A Eming
Feb 6, 2007·Clinics in Dermatology·Sabine A EmingJeffrey M Davidson
May 23, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rie UtohKatsutoshi Yoshizato
Jun 1, 2013·Journal of Tissue Engineering and Regenerative Medicine·Rainer MittermayrHeinz Redl
May 19, 2004·American Journal of Surgery·Pablo A Jimenez, Sydney E Jimenez
Nov 28, 2006·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Russell R ReidThomas A Mustoe
Jun 26, 2003·The Surgical Clinics of North America·Nicola C PetrieElof Eriksson
Jan 15, 2015·Plastic and Reconstructive Surgery. Global Open·Zhong ZhengChia Soo
May 16, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·L B NanneyJ M Davidson
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·F Yao, E Eriksson
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·T M Crombleholme
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·L A ChandlerM L Phillips
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K R Cutroneo, J F Chiu
Jul 17, 2012·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·C I Günter, H-G Machens
Jul 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gulsun ErdagJeffrey R Morgan
Jun 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bharat G BajajStelios T Andreadis

❮ Previous
Next ❯

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