Genetics of abnormal human fertilization

Human Reproduction
Gianpiero D PalermoZev Rosenwaks

Abstract

The purpose of this study was to assess the genetic status of abnormal zygotes following assisted fertilization. Dispermic, monopronucleated and digynic zygotes were allowed to cleave intact or after enucleation, and on the biopsied blastomeres, multiplex polymerase chain reaction and fluorescent in-situ hybridization were performed. It was found that the distal pronucleus was usually male in origin in dispermic embryos, and that the sex ratio was restored when they were enucleated; however, they became mosaic at metaphase and their genetic heterogeneity was not restored after enucleation. Monopronucleated zygotes derived from standard in-vitro insemination can be transferred to the patient, since they usually showed normal diploid complement in their cells. On the contrary, single-pronucleated zygotes derived from intracytoplasmic sperm injection were usually activated parthenogenetically, but not fertilized. Digynic embryos, unlike dispermic ones, had a very low incidence of mosaicism, and when present, such mosaicism originated at a later embryo division. Most of the digynic embryos were triploid, indicating that the first division was normal and bipolar; moreover, when the female pronucleus was removed, they became diploid ...Continue Reading

Citations

Oct 1, 1996·Molecular Reproduction and Development·C M O'TooleL R Fraser
Aug 5, 1998·American Journal of Reproductive Immunology : AJRI·E S SillsG D Palermo
Nov 3, 2012·Journal of Pathogens·Sonia Gupta, Vijay Prabha
Sep 24, 2005·Reproductive Biomedicine Online·Takumi TakeuchiGianpiero D Palermo
Nov 27, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Qing HuanZi-Jiang Chen
Dec 2, 2014·PloS One·Gianpiero D PalermoZev Rosenwaks
Sep 24, 2015·Journal of Assisted Reproduction and Genetics·Yoshiteru KaiYasuyuki Mio
Aug 25, 1999·Molecular Human Reproduction·M MoomjyG D Palermo
Jan 5, 2016·Reproductive Medicine and Biology·Kyoko Iwata, Yasuyuki Mio
Nov 7, 2020·Journal of Assisted Reproduction and Genetics·Mounia HaddadGianpiero D Palermo

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