DOI: 10.1101/488486Dec 5, 2018Paper

Genetics of adaptation of the ascomycetous fungus Podospora anserina to submerged cultivation

BioRxiv : the Preprint Server for Biology
Olga A KudryavtsevaAlexey S Kondrashov

Abstract

Podospora anserina is a model ascomycetous fungus which shows pronounced phenotypic senescence when grown on solid medium but possesses unlimited lifespan under submerged cultivation. In order to study the genetic aspects of adaptation of P. anserina to submerged cultivation, we initiated a long-term evolution experiment. In the course of the first four years of the experiment, 125 single-nucleotide substitutions and 23 short indels were fixed in eight independently evolving populations. Six proteins that affect fungal growth and development evolved in more than one population; in particular, the G-protein alpha subunit FadA evolved in seven out of eight experimental populations. Parallel evolution at the level of genes and pathways, an excess of nonsense and missense substitutions, and an elevated conservation of proteins and their sites where the changes occurred suggest that many of the observed allele replacements were adaptive and driven by positive selection.

Related Concepts

Alleles
Biological Evolution
Fungi
Genes
Guanosine
Nucleotides
Site
Adaptation
Solid state
Missense Mutation

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