Genetics of resistance to photobacteriosis in gilthead sea bream (Sparus aurata) using 2b-RAD sequencing

BMC Genetics
Muhammad L AslamTheo H E Meuwissen

Abstract

Photobacteriosis is an infectious disease developed by a Gram-negative bacterium Photobacterium damselae subsp. piscicida (Phdp), which may cause high mortalities (90-100%) in sea bream. Selection and breeding for resistance against infectious diseases is a highly valuable tool to help prevent or diminish disease outbreaks, and currently available advanced selection methods with the application of genomic information could improve the response to selection. An experimental group of sea bream juveniles was derived from a Ferme Marine de Douhet (FMD, Oléron Island, France) selected line using ~ 109 parents (~ 25 females and 84 males). This group of 1187 individuals represented 177 full-sib families with 1-49 sibs per family, which were challenged with virulent Phdp for a duration of 18 days, and mortalities were recorded within this duration. Tissue samples were collected from the parents and the recorded offspring for DNA extraction, library preparation using 2b-RAD and genotyping by sequencing. Genotypic data was used to develop a linkage map, genome wide association analysis and for the estimation of breeding values. The analysis of genetic variation for resistance against Phdp revealed moderate genomic heritability with estim...Continue Reading

References

Aug 27, 1998·American Journal of Human Genetics·K W BromanJ L Weber
Sep 5, 2006·Genetics·Rafaella FranchLuca Bargelloni
Aug 4, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N PiyasatianJ C M Dekkers
Oct 15, 2008·PloS One·Nathan A BairdEric A Johnson
Oct 24, 2008·Journal of Dairy Science·P M VanRaden
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Dec 8, 2009·Genetics·Aaron K WongMark W Neff
Dec 21, 2010·American Journal of Human Genetics·Jian YangPeter M Visscher
Mar 17, 2011·European Journal of Human Genetics : EJHG·Jian YangUNKNOWN GIANT Consortium
May 25, 2011·BMC Bioinformatics·David HabierDorian J Garrick
May 23, 2012·Nature Methods·Shi WangMikhail V Matz
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Jul 2, 2014·Journal of Immunology Research·Francesca Andreoni, Mauro Magnani
Jul 11, 2014·Genetics·Paulino Pérez, Gustavo de los Campos
Aug 6, 2014·Development, Growth & Differentiation·Maha AnaniTetsuya Taga
Oct 14, 2014·Marine Genomics·Costas S TsigenopoulosGeorgios Kotoulas
Oct 17, 2014·Molecular Ecology·Jonathan B PuritzChristopher E Bird
Oct 31, 2014·G3 : Genes - Genomes - Genetics·Nathan R CampbellShawn R Narum
Sep 5, 2015·Genetics, Selection, Evolution : GSE·Christos PalaiokostasMarc Vandeputte
Jul 1, 2016·Genetics, Selection, Evolution : GSE·Hsin-Yuan TsaiRoss D Houston
Sep 23, 2016·G3 : Genes - Genomes - Genetics·Christos PalaiokostasLuca Bargelloni
May 5, 2017·Proceedings. Biological Sciences·Jessica K AbbottBengt Hansson
Feb 7, 2018·Frontiers in Immunology·Justyna SobocińskaKatarzyna Kwiatkowska

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Methods Mentioned

BETA
genotyping
electrophoresis
PCR
Assay

Software Mentioned

CERVUS
GBLUP
Perl
bcftools
R
2bRAD pipeline
OrderMarkers
HIT
BGLR
NextSeq

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