Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications

Journal of Biomedical Materials Research. Part a
Le-Ping YanRui L Reis

Abstract

In this study, genipin-cross-linked collagen/chitosan biodegradable porous scaffolds were prepared for articular cartilage regeneration. The influence of chitosan amount and genipin concentration on the scaffolds physicochemical properties was evaluated. The morphologies of the scaffolds were characterized by scanning electron microscope (SEM) and cross-linking degree was investigated by ninhydrin assay. Additionally, the mechanical properties of the scaffolds were assessed under dynamic compression. To study the swelling ratio and the biostability of the collagen/chitosan scaffold, in vitro tests were also carried out by immersion of the scaffolds in PBS solution or digestion in collagenase, respectively. The results showed that the morphologies of the scaffolds underwent a fiber-like to a sheet-like structural transition by increasing chitosan amount. Genipin cross-linking remarkably changed the morphologies and pore sizes of the scaffolds when chitosan amount was less than 25%. Either by increasing the chitosan ratio or performing cross-linking treatment, the swelling ratio of the scaffolds can be tailored. The ninhydrin assay demonstrated that the addition of chitosan could obviously increase the cross-linking efficiency. T...Continue Reading

References

Aug 1, 1989·Journal of Biomedical Materials Research·B C LiuM Spira
May 14, 1993·Science·R Langer, J P Vacanti
Nov 1, 1995·Journal of Biomedical Materials Research·K S WeadockM G Dunn
Jul 1, 1997·Journal of Biomedical Materials Research·S NehrerM Spector
Nov 25, 1998·Journal of Biomedical Materials Research·H W SungC T Chiu
Nov 25, 1998·Journal of Biomedical Materials Research·L L HuangD M Huang
Nov 9, 2000·Biomaterials·D W Hutmacher
Jun 9, 2001·International Journal of Pharmaceutics·C H LeeY Lee
May 8, 2003·Journal of Biomedical Materials Research. Part a·Huang-Chien LiangHsing-Wen Sung
Nov 12, 2003·Biomaterials·A SionkowskaT J Wess
Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·A K LynnW Bonfield
Nov 2, 2004·Journal of Materials Science. Materials in Medicine·R M SilvaR L Reis
May 17, 2005·Biomaterials·Alberto Di MartinoMakarand V Risbud
Jan 31, 2006·IEE Proceedings. Nanobiotechnology·D J Williams, I M Sebastine
May 17, 2006·Acta Biomaterialia·Dominique J GriffonAnn L Johnson
Feb 27, 2007·Journal of Materials Science. Materials in Medicine·J T OliveiraR L Reis
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Valeria ChionoPaolo Giusti
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·J F Mano, R L Reis
Sep 26, 2008·Biomacromolecules·Simone S SilvaClaudio Migliaresi

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Citations

Jun 8, 2011·ACS Applied Materials & Interfaces·Yu-Jer HwangJulia G Lyubovitsky
Apr 26, 2013·Tissue Engineering. Part a·Jingjing ZhangZigang Ge
May 1, 2013·Regenerative Medicine·Ken YeDamian E Myers
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Melika SaremV Prasad Shastri
Jul 6, 2014·AAPS PharmSciTech·Naeema MayetViness Pillay
Nov 17, 2015·International Journal of Molecular Sciences·Alessandro R ZorziJoão B Miranda
Oct 18, 2012·Acta Biomaterialia·Antonio FranceskoTzanko Tzanov
Jan 23, 2016·Carbohydrate Polymers·Kevin Barrett MilesHoward William Trevor Matthew
Jan 24, 2012·Journal of Bioscience and Bioengineering·Shuaijun JiaJian Liu
Dec 22, 2015·Carbohydrate Polymers·Kalirajan CheirmaduraiRagothaman Murali
Aug 8, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Jing ZhangShulin Yang
Mar 15, 2016·International Journal of Biological Macromolecules·Natascia CozzaClaudio Migliaresi
Nov 23, 2015·Colloids and Surfaces. B, Biointerfaces·Guangqian LanDayang Wu
Nov 18, 2015·Carbohydrate Polymers·Jinlei LiShuguang Yang
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Supaporn SangkertWen Lin Chai
Oct 11, 2015·International Journal of Pharmaceutics·Suguna Selvakumaran, Ida Idayu Muhamad
Oct 28, 2015·BioMed Research International·Martin Rodríguez-VázquezLuis Fernando Quiñones-Olvera
May 6, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Megan E McGannDiane R Wagner
Apr 29, 2014·Journal of Biomedical Materials Research. Part a·Tatiana C Gamboa-MartínezGloria Gallego-Ferrer
Aug 29, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·L-P YanR L Reis
Sep 1, 2015·International Journal of Biological Macromolecules·Jorge PadrãoVitor Sencadas
Jun 6, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Nadeem SiddiquiEsmaiel Jabbari
May 9, 2012·International Journal of Biological Macromolecules·Jixiang ZhuJun Wan
Jul 1, 2012·Carbohydrate Polymers·Riccardo A A MuzzarelliAntonio Gigante
Apr 29, 2016·Journal of Biomaterials Science. Polymer Edition·Zihao ChenJimin Wu
Dec 25, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jamila GittensJohn G Kennedy
Mar 31, 2017·Journal of Materials Science. Materials in Medicine·Mônica Helena Monteiro do NascimentoChristiane Bertachini Lombello

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