Genipin induces cell death via intrinsic apoptosis pathways in human glioblastoma cells

Journal of Cellular Biochemistry
Narges AhaniMajid Alipour Eskandani

Abstract

Genipin, a compound derived from Gardenis jasminoides Ellis fruits, was demonstrated to be the specific uncoupling protein 2 (UCP2) inhibitor. UCP2 is a mitochondrial carrier protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from adenosine triphosphate (ATP) synthesis. Several studies revealed that UCP2 is broadly over-expressed in leukemia, colorectal, lung, ovarian, prostate, testicular, and bladder cancers. However, the effect of genipin still needs to be elucidated in neurological malignancies. In this study, we investigated the anticancer effect of genipin in U87MG and A172 cell lines. The anticancer effect of genipin on these cell lines was measured by microculture tetrazoliumtest (MTT), Trypan blue exclusion, and colony formation assays, in the presence of various concentrations of genipin at different time intervals. We assessed apoptosis and measure intracellular reactive oxygen species (ROS) by flow cytometry. Expression of UCP2 and some of the genes involved in apoptosis was analyzed by real-time quantitative polymerase chain reaction (PCR). Results of the MTT assay showed that genipin moderately reduced metabolic activity of both cell lines in dose- ...Continue Reading

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Citations

Dec 5, 2019·Anais Da Academia Brasileira De Ciências·Juliana M SoaresLuciana F Romão
Feb 15, 2020·Journal of Biomedical Materials Research. Part a·Sarah BonnesœurBéatrice Labat
Jan 17, 2021·Life Sciences·Nastaran InjinariHossein Teimori
Jul 4, 2021·Colloids and Surfaces. B, Biointerfaces·Güliz AkŞenay Hamarat Şanlıer
Dec 29, 2019·Food Research International·Iramaia Angelica Neri-NumaGlaucia Maria Pastore

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