Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells

Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy
Chunyan LiuJianhui Liu

Abstract

To investigate the effect of geniposide on the biosynthesis of insulin and the expression protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin 1 (ERO1) in the presence of low (5 mM) and high (25 mM) glucose in pancreatic β cells. The content of insulin was measured by ELISA, the number of SH groups was determined with the classical chromogenic reagent, 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB; also known as Ellman's reagent), the expressions of PDI and ERO1 were analyzed by Western blot. Geniposide played contrary roles on the accumulation of H2O2, the ratio of GSH/GSSG and the thiol-disulfide balance in the presence of low (5 mM) and high (25 mM) glucose in rat pancreatic INS-1 cells. Geniposide also regulated the protein levels of protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin1 (ERO1), the two key enzymes for the production of H2O2 during the biosynthesis of insulin in INS-1 cells. Geniposide affects glucose-stimulated insulin secretion by modulating the thiol-disulfide balance that is controlled by the redox signaling in pancreatic β cells.

References

Feb 1, 1993·Free Radical Biology & Medicine·P C PanusB A Freeman
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·T NakanoK Ishizaka
Sep 25, 1999·The Journal of Endocrinology·B AkessonI Lundquist
Mar 12, 2002·Methods in Enzymology·Dean P Jones
Oct 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Ingmar LundquistBo Hellman
Jul 16, 2004·The Journal of Biological Chemistry·Seema Chakravarthi, Neil J Bulleid
Jul 19, 2005·Biochemical and Biophysical Research Communications·Gábor NardaiPéter Csermely
Nov 23, 2011·The Journal of Biological Chemistry·Gautam RajpalPeter Arvan
Oct 5, 2012·The Journal of Biological Chemistry·Ryan J MaillouxMary-Ellen Harper
Mar 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adam M BenhamIneke Braakman
Mar 4, 2014·Lancet·James D Watson
Feb 6, 2015·Free Radical Biology & Medicine·Ester Zito
Jul 15, 2015·Biochemical and Biophysical Research Communications·Xiaojing LiuJiarui Wu
Oct 22, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniela ElettoYair Argon
Mar 22, 2016·Experimental and Therapeutic Medicine·Ypatios SpanidisDemetrios Kouretas
Dec 22, 2016·Journal of Endocrinological Investigation·C Y LiuJ H Liu
Apr 2, 2017·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·L X GuoK Y Tam
Feb 16, 2019·Pflügers Archiv : European journal of physiology·Israa Mohammad Al-AmilyAlbert Salehi

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Methods Mentioned

BETA
dissecting
protein assay
ELISA

Software Mentioned

Origin
Quantity One

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