Geniposide Suppresses Hepatic Glucose Production via AMPK in HepG2 Cells

Biological & Pharmaceutical Bulletin
Lixia GuoZhongyi Yin

Abstract

Geniposide is one of the main compounds in Gardenia jasminoides ELLIS and has many pharmacological activities, but its anti-hyperglycemic activity has not yet been fully explored. This study was designed to determine, for the first time, how geniposide from G. jasminoides regulates hepatic glucose production, and the underlying mechanisms. During in vitro study, we found the inhibitory effect of geniposide on the hepatic glucose production is partly through AMP-activated protein kinase (AMPK) activation in HepG2 cells. Geniposide significantly inhibited hepatic glucose production in a dose-dependent manner. AMPK, acetyl coenzyme A synthetase (ACC) and forkhead box class O1 (FoxO1) phosphorylation were stimulated by different concentrations of geniposide. In addition, the enzyme activities of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) were all significantly suppressed. What is important is that these effects were partly reversed by (1) inhibition of AMPK activity by compound C, a selective AMPK inhibitor, and by (2) suppression of AMPKα expression by small interfering RNA (siRNA). In summary, geniposide potentially ameliorates hyperglycemia through inhibition of hepatic gluconeogenesis by modula...Continue Reading

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Citations

Oct 11, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mingqiu ShanSam Fong Yau Li
Apr 7, 2018·BMC Complementary and Alternative Medicine·Ennian LengWenna Li
Sep 19, 2019·Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry·Humaira B NoorMd A T Sagor
Dec 22, 2016·Journal of Endocrinological Investigation·C Y LiuJ H Liu
Apr 23, 2020·Oxidative Medicine and Cellular Longevity·Maciej DanielewskiTomasz Sozański
May 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yan-Xi ZhouCheng Peng
Apr 15, 2018·International Immunopharmacology·Xiaolei HuXiaomei Zhang

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