Genistein and daidzein induce apoptosis of colon cancer cells by inhibiting the accumulation of lipid droplets

Food & Nutrition Research
Yu-Si LiangAike Li

Abstract

The purpose of this study was to investigate the possible mechanisms of genistein (GEN) and daidzein (DAI) in inducing apoptosis of colon cancer cells by inhibition of lipid droplets (LDs) accumulation. HT-29 cells were used and treated by GEN or DAI in this paper. LDs accumulation was induced and inhibited by oleic acid (OA) and C75, respectively. The expression changes of LDs-related markers were confirmed by semiquantitative real time-PCR (RT-PCR), Western blotting, and immunofluorescence staining. GEN and DAI effectively reduced the LDs accumulation and downregulated the expression of Perilipin-1, ADRP and Tip-47 family proteins and vimentin levels. GEN and DAI significantly induced the mRNA expression of PPAR-γ, Fas, FABP, glycerol-3-phosphate acyltransferase (GPAT3), and microsomal TG transfer protein (MTTP), and reduced the mRNA expression of UCP2. Furthermore, the results showed a decrease of PI3K expression by GEN and DAI when compared with OA treatment, and both GEN and DAI can increase the expression of FOXO3a and caspase-8 significantly when these proteins were decreased by OA treatment. GEN is more effective than DAI in inducing cell apoptosis. Our results demonstrated that GEN and DAI inhibit the accumulation of L...Continue Reading

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Citations

Nov 15, 2018·International Journal of Molecular Sciences·Salvatore ChirumboloTaras Upyr
Feb 18, 2020·International Journal of Cancer. Journal International Du Cancer·Bernhard EnglingerWalter Berger
Feb 23, 2020·Pharmaceutics·Anna KwiecieńMaria Walczak
May 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yae Rim ChoiMin Jung Kim
Oct 22, 2020·Medicinal Research Reviews·Ana T RufinoEduarda Fernandes
Apr 6, 2021·International Journal of Food Science·Aparna Bettaiah, Hema Bommanamane Prabhushankar

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Methods Mentioned

BETA
biopsies
enzyme-linked immunosorbent assay
scanning electron microscopy
flow cytometry

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