Genistein and Myd88 Activate Autophagy in High Glucose-Induced Renal Podocytes In Vitro

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Yuanyuan WangYing Liu

Abstract

BACKGROUND Renal podocyte damage plays a crucial role in the development of diabetic nephropathy. Genistein is derived from a leguminous plant, and MyD88 and TRIF are adaptor molecules in the Toll-like receptor (TLR) signaling pathway, which may play a role in autophagy. In this study, we utilized an in vitro high glucose (HG)-treated podocyte model to investigate the effects and underlying mechanisms of Genistein and MyD88 or TRIF siRNA induced autophagy and renal protection. MATERIAL AND METHODS An immortalized mouse podocyte cell line was treated with HG, Genistein, chloroquine, and/or transfected with specific Myd88 and TRIF siRNAs. The formation of autophagosomes and related autophagic vacuoles were monitored by transmission electron microscopy. The expression of autophagy-related factors and podocyte structure and functional markers, including LC3, p62, p-mTOR, synaptopodin, and nephrin, were measured by Western blot, and LC3 and p-mTOR expression were also assessed by immunofluorescence. RESULTS We showed that HG transiently (after 6-h exposure) induced expression of the autophagy activation marker LC3-II in podocytes. Genistein treatment induced autophagy in both normal and HG-treated podocytes through inactivating mTOR...Continue Reading

Citations

Sep 7, 2019·BMC Complementary and Alternative Medicine·Yan ChenWeisheng Feng
Aug 23, 2020·International Journal of Molecular Sciences·Sara Balbuena-PecinoIsabel Navarro
Sep 17, 2020·International Journal of Molecular Sciences·Sebastian MertowskiWojciech Załuska
Nov 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Liyan YangYangbin Pan
Aug 9, 2021·Chinese Medicine·Qichao HuYanLing Zhao

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Methods Mentioned

BETA
electrophoresis
transmission electron microscopy

Software Mentioned

Image J
SPSS

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