Genistein Antagonizes 17β-Estradiol Effects on Glutamate-Evoked Masseter Muscle Hypernociception in Rats

Frontiers in Neurology
Hui-Fei JieQiu-Fei Xie

Abstract

Temporomandibular disorders (TMDs) predominantly affect women of reproductive ages, with pain as the main symptom. The aim of the present study was to examine the effects of 17β-estradiol (E2) on glutamate-evoked hypernociception of masseter muscle and to examine whether genistein could antagonize the effects of E2 in female rats. Injection of glutamate into the masseter muscle dose-dependently decreased head withdrawal thresholds, a parameter for mechanical hypernociception. Head withdrawal thresholds in ovariectomized rats also decreased with increasing doses of E2 replacement, and were further aggravated by injection of glutamate (1M, 40μL) into the masseters. Genistein at doses of 7.5 and 15 mg/kg antagonized E2-induced hypernociception of masseter muscle, and at doses of 7.5, 15, and 30 mg/kg also antagonized E2 potentiation of glutamate-evoked hypernociception of masseter muscle. Genistein produced optimal antagonistic effects of E2 on nociception behavior at a dose of 15 mg/kg. On the molecular level, tyrosine phosphorylation of the NR2B subunit of the N-methyl-D-aspartate receptor (pNR2B) and phosphorylated mitogen-activated protein kinase (pERK1/2) were significantly upregulated in the hippocampus following glutamate i...Continue Reading

References

Jan 22, 2002·Neuroscience·B E CairnsC B Berde
Sep 19, 2003·Phytotherapy Research : PTR·Andreana L Ososki, Edward J Kennelly
Sep 25, 2004·The Journal of Pharmacology and Experimental Therapeutics·Naohito ShimoyamaCharles E Inturrisi
Nov 18, 2005·Experimental Brain Research·Brian E CairnsLars Arendt-Nielsen
Nov 13, 2007·Archives of Oral Biology·G BejatB J Sessle
Mar 4, 2008·Archives of Oral Biology·Eduardo E CastrillonPeter Svensson
Jan 20, 2009·Brain Research Reviews·Ru-Rong JiGary R Strichartz
Feb 13, 2009·Cephalalgia : an International Journal of Headache·C S LivermanN E J Berman
Oct 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Long-Jun Wu, Min Zhuo
Mar 23, 2010·Neuropharmacology·Renqi HuangGlenn H Dillon
Jul 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yu-Wei WuXu-Chen Ma
Jul 16, 2010·The European Journal of Neuroscience·Xue Jun Liu, Michael W Salter
Oct 6, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Luciano Mendoza-GarcésMarco Cerbón
Nov 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Deepak P SrivastavaFeng Liu
May 9, 2012·The Journal of Pain : Official Journal of the American Pain Society·Yu-Wei WuXu-Chen Ma
Jul 7, 2012·Behavioural Brain Research·Guy SandnerAyikoe Guy Mensah-Nyagan
Feb 28, 2014·International Journal of Molecular Sciences·Weifeng HuangQi Luo
Jul 10, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Xiao-Xing KouYe-Hua Gan
Jan 31, 2015·Journal of Oral & Facial Pain and Headache·Abhishek KumarPeter Svensson

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
hormone replacement therapy
transfection

Software Mentioned

ImageJ
SPSS
GraphPad
Prism7

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