Genistein-induced differentiation of breast cancer stem/progenitor cells through a paracrine mechanism.

International Journal of Oncology
Yanchen LiuXinmei Kang

Abstract

It is believed that breast cancer stem cells (BCSCs), like normal stem cell counterparts, have the capacity of self-renewal and differentiation. Simultaneously, estrogen receptor (ER)-negative (-) BCSCs are affected by surrounding differentiated ER-positive (+) tumor cells by virtue of paracrine signaling within the tumor micro-environment. Genistein (GEN), as a sort of phytoestrogen, can act on ER+ breast cancer cells but the role of GEN in the differentiation of neighboring ER- BCSCs has not been defined. Transwell co-culture system was utilized so as to elaborate the interaction between well-differentiated ER+ breast cancer cells (MCF-7) and ER- breast cancer stem/progenitor cells (mammospheres derived from MDA-MB-231 cells). GEN-induced differentiation of BCSCs was analyzed by mammospheres formation assay, flow cytometry and RT-PCR after a 3 day solo-culture or co-culture. We find that GEN sized 2 µM, and 40 nM, effectively promotes morphological alteration of mammospheres, reduces the ratio of subset of CD44+/CD24-/ESA+ cells and upregulates the expression of differentiated cell markers of mammospheres in co-culture system, but not in solo-culture condition. Besides, we demonstrate that the differentiation-inducing effect ...Continue Reading

References

Aug 26, 2000·Cytokine & Growth Factor Reviews·T Akiyama
Aug 22, 2002·Carcinogenesis·Anna H WuMalcolm C Pike
Dec 3, 2002·Nature Reviews. Genetics·Haifan Lin
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
May 21, 2003·Genes & Development·Gabriela DontuMax S Wicha
Jun 30, 2004·Trends in Endocrinology and Metabolism : TEM·Gabriela DontuMax S Wicha
Jan 27, 2005·Stem Cells·Adnan Z Rizvi, Melissa H Wong
Sep 21, 2005·Endocrine-related Cancer·Daniel Medina
Sep 27, 2005·Journal of the American Dietetic Association·Carolyn Y FangMary B Daly
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Jan 4, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Martijn VerheusPetra H M Peeters
Mar 14, 2007·Cancer Cell·Michail ShipitsinKornelia Polyak
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Laura CiarloniCathrin Brisken
Sep 15, 2007·CA: a Cancer Journal for Clinicians·Christine DuffyAnn Partridge
Mar 5, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Motoki IwasakiUNKNOWN Japan Public Health Center-based prospective study group
May 1, 2008·Journal of the National Cancer Institute·Xiaoxian LiJenny C Chang
May 20, 2008·Cancer Letters·Colleen Wu, Benjamin A Alman
Sep 12, 2008·Nature Reviews. Cancer·Jane E Visvader, Geoffrey J Lindeman
Dec 31, 2008·Cell Research·Tetsuro Watabe, Kohei Miyazono
Mar 5, 2009·Journal of Mammary Gland Biology and Neoplasia·Bhuvanesh Dave, Jenny Chang
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chad J CreightonJenny C Chang
May 7, 2010·Nature·Purna A JoshiRama Khokha
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christine M FillmoreCharlotte Kuperwasser
Jul 16, 2011·Hormones & Cancer·Ciara S O'BrienRobert B Clarke
Jun 19, 2013·Cancer Prevention Research·Fitriya N DewiJ Mark Cline
Oct 15, 2013·PloS One·Chandrani MukhopadhyayMayumi Naramura
Jul 1, 2014·Climacteric : the Journal of the International Menopause Society·F-P Chen, M-H Chien

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Citations

Jul 26, 2019·Phytotherapy Research : PTR·Qi ZhangHong-Yu Han
Dec 14, 2018·Clinical Science·Luis MolinaPamela Ehrenfeld
Jan 19, 2018·Oncology Letters·Yu WangZhitu Zhu
Jan 12, 2019·Critical Reviews in Food Science and Nutrition·Hao-Feng GuMin Du
Aug 3, 2021·Oxidative Medicine and Cellular Longevity·Javad Sharifi-RadDaniela Calina
Jul 18, 2021·Seminars in Cancer Biology·Man ChuZhi-Wei Wang

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