Abstract
Genistein is a bioflavonoid enriched in soy products. However, high levels of maternal soy consumption have been linked to the development of infant leukemia ALL and AML. The majority of infant leukemia is linked to mixed lineage leukemia gene (MLL) translocations. Previous studies have implicated topoisomerase II (Top2) in genistein-induced infant leukemia. In order to understand the roles of the two Top2 isozymes in and the molecular mechanism for genistein-induced infant leukemia, we carried out studies in vitro using purified recombinant human Top2 isozymes, as well as studies in cultured mouse myeloid progenitor cells (32Dc13) and Top2beta knockout mouse embryonic fibroblasts (MEFs). First, we showed that genistein efficiently induced both Top2alpha and Top2beta cleavage complexes in the purified system as well as in cultured mouse cells. Second, genistein induced proteasomal degradation of Top2beta in 32Dc13 cells. Third, the genistein-induced DNA double-strand break (DSB) signal, gamma-H2AX, was dependent on the Top2beta isozyme and proteasome activity. Fourth, the requirement for Top2beta and proteasome activity was mirrored in genistein-induced DNA sequence rearrangements, as monitored by a DNA integration assay. Toget...Continue Reading
References
Oct 26, 1984·Science·K M TeweyL F Liu
Aug 4, 1995·Biochemical and Biophysical Research Communications·M GovoniA Pession
Jul 1, 1997·Molecular and Cellular Biology·M StanullaP D Aplan
Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Austin, K L Marsh
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·R StrickJ D Rowley
Jun 15, 2001·The Journal of Biological Chemistry·S P Sim, L F Liu
Sep 8, 2001·The Journal of Biological Chemistry·Y MaoL F Liu
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·James C Wang
Mar 16, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Logan G SpectorJulie A Ross
Sep 30, 2005·Leukemia·C J BettiA T M Vaughan
Jun 24, 2006·Science·Bong-Gun JuMichael G Rosenfeld
Jul 5, 2006·Molecular Cancer Therapeutics·Mobeen MalikJohn L Nitiss
Aug 9, 2006·DNA Repair·Yanming Zhang, Janet D Rowley
Sep 16, 2006·The Journal of Biological Chemistry·Ailing ZhangLeroy F Liu
Sep 20, 2006·Cancer Research·Eszter S HarsLeroy F Liu
Jan 20, 2007·Gene·Omar L Kantidze, Sergey V Razin
May 1, 2007·Carcinogenesis·Sahar Barjesteh van Waalwijk van Doorn-KhosrovaniFrederik J van Schooten
Feb 21, 2008·Archives of Biochemistry and Biophysics·Barry Halliwell
Jan 1, 2009·PloS One·Kuniaki SanoKen Tsutsui
Citations
Jul 26, 2012·International Journal of Environmental Research and Public Health·Ian G Cowell, Caroline A Austin
Dec 20, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel SilvaMozart Marins
Apr 3, 2012·The International Journal of Biochemistry & Cell Biology·Anna Vávrová, Tomáš Šimůnek
May 17, 2011·Pharmacology & Therapeutics·Beatrice MercorelliArianna Loregian
Nov 20, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mahshid HodjatMohammad Abdollahi
Aug 4, 2015·Plant Physiology and Biochemistry : PPB·Aneta ŻabkaJanusz Maszewski
Apr 27, 2011·Molecular Nutrition & Food Research·Faiza M KalfalahFritz Boege
Sep 23, 2014·Food Chemistry·Seiichi SakamotoSatoshi Morimoto
Jul 11, 2013·The American Journal of Pathology·Yi-Wen WangNan-Haw Chow
Dec 17, 2016·Molecular Nutrition & Food Research·Varinderpal S DhillonMichael Fenech
Jan 15, 2017·Plant Physiology and Biochemistry : PPB·Aneta ŻabkaJanusz Maszewski
Nov 6, 2018·Environmental and Molecular Mutagenesis·Bhawana BariarChristine Richardson
Aug 26, 2020·Molecular and Cellular Biochemistry·Begum DariyaMadhu Sudhana Saddala
Apr 4, 2017·Journal of Neuroscience Research·Ying LiLi Cai
Feb 20, 2015·Nature Reviews. Cancer·Mohamed E AshourSherif F El-Khamisy
May 26, 2017·Archives of Toxicology·Olavi PelkonenUNKNOWN EFSA WG EPI1 and its other members
Aug 23, 2019·DNA Repair·Karin C NitissLeslyn A Hanakahi
Aug 27, 2019·DNA Repair·Maryam MajidiniaBahman Yousefi
Feb 18, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Adrià Botet-CarrerasPatricia Horcajada
Jun 12, 2019·Bioorganic & Medicinal Chemistry Letters·Esha D DalvieNeil Osheroff