Genistein inhibits high fat diet-induced obesity through miR-222 by targeting BTG2 and adipor1

Food & Function
Mailin GanLi Zhu

Abstract

Obesity and diabetes mellitus have become major health problems worldwide. In recent years, genistein has been found to be capable of inhibiting obesity and alleviating insulin resistance. However, the molecular mechanism of genistein against obesity is still not fully understood. In this study, we used 3T3-L1 preadipocytes and obese mice as models to explore the molecular mechanism of genistein against obesity. We found that genistein can inhibit obesity and downregulate the expression of miR-222 in mouse adipose tissue. In 3T3-L1 preadipocytes, treatment with miR-222 inhibitor or genistein reduced the expression of miR-222 and promoted lipid decomposition, while miR-222 treatment increased the expression of miR-222 and inhibited lipolysis. Moreover, the dual-luciferase reporter assay system confirmed that BTG2 and adipor1 are the target genes of miR-222. Experiments conducted in vitro and in vivo suggest that genistein may regulate lipid metabolism in the adipose tissue of obese mice by regulating the expression of miR-222 and its target genes, BTG2 and adipor1. Our findings provide a new epigenetic mechanism underpinning the ability of genistein to resist obesity. These results may provide a reference point for the dietary t...Continue Reading

References

Feb 14, 1998·Hypertension·I GiménezJ O Alda
May 29, 2003·Current Pharmaceutical Design·L K HeilbronnE Ravussin
Jul 17, 2003·Endocrinology·Afia NaazPaul S Cooke
Oct 4, 2005·Lancet·David W Haslam, W Philip T James
May 22, 2008·Cancer Letters·Sanjeev BanerjeeFazlul H Sarkar
Jun 13, 2008·The Journal of Nutritional Biochemistry·Hea Jin ParkClifton A Baile
Aug 22, 2008·Kidney International·Mi Jeong SungSung Kwang Park
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Nov 28, 2012·European Journal of Pharmacology·Nouredine Behloul, Guanzhong Wu
Sep 26, 2013·Clinical Science·Aaron D McClelland, Phillip Kantharidis
May 23, 2014·Cellular Signalling·Yongdong PengSiwen Jiang
Feb 24, 2015·Trends in Endocrinology and Metabolism : TEM·Birgit GustafsonUlf Smith
Jan 8, 2016·Molecular and Cellular Biochemistry·Suji KimKye Won Park
Jun 12, 2016·Journal of Translational Medicine·Hoon Suk ParkCheol Whee Park
Nov 14, 2016·Hormones : International Journal of Endocrinology and Metabolism·Anastasia MarkakiAspasia Spyridaki
Mar 1, 2017·Nutrition·Kyria Jayanne Clímaco CruzDilina do Nascimento Marreiro PhD
Oct 27, 2017·Critical Reviews in Oncology/hematology·Vidya MukundAfroz Alam
Nov 25, 2017·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Mailin GanLi Zhu
Mar 7, 2018·In Vitro Cellular & Developmental Biology. Animal·Mailin GanLi Zhu
Apr 29, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Olga GulyaevaHei Sook Sul
Feb 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mailin GanLi Zhu

❮ Previous
Next ❯

Citations

Jan 1, 2021·International Journal of Molecular Sciences·Alina Kuryłowicz
Jul 3, 2021·International Journal of Molecular Sciences·Adriana FodorAngela Cozma
Jul 3, 2021·International Journal of Molecular Sciences·Shiyao ZhangSiyu Chen
Aug 25, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mehdi ZobeiriMohammad Abdollahi

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
transfection
Fluorescence

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved