Genistein-triggered anticancer activity against liver cancer cell line HepG2 involves ROS generation, mitochondrial apoptosis, G2/M cell cycle arrest and inhibition of cell migration

Archives of Medical Science : AMS
Qian ZhangJiehua Yang

Abstract

Liver cancer is one of the most common malignancies across the globe and one of the major causes of cancer-related mortality. With limited available treatment options, there is an urgent need to look for new available options. Genistein is an important plant flavonoid and has been shown to possess tremendous pharmacological potential. The objective of the present study was therefore to evaluate the anticancer effect of the genistein. The antiproliferative activity and IC50 of genistein were determined by MTT assay. Reactive oxygen species (ROS) and cycle distribution were investigated by flow cytometry. Apoptosis was detected by DAPI and annexin V/IP staining. Cell migration was investigated by wound healing assay. Protein expression was estimated by western blotting. MTT assay revealed that genistein reduced the cell viability of HepG2 cancer cells in a dose-dependent manner. Genistein also reduced the colony forming potential of the HepG2 cell concentration dependently. The IC50 of genistein was found to be 25 μM. Genistein caused G2/M cell cycle arrest and G2/M cells increased from 4.2% in the control to 56.4% at 100 μM concentration. Genistein prompted generation of significant (p < 0.01) amounts of ROS, ultimately favourin...Continue Reading

Citations

Oct 12, 2019·International Journal of Molecular Sciences·Alok RanjanSanjay K Srivastava
Oct 1, 2020·International Journal of Molecular Sciences·Carlos Rafael-VidalJose M Pego-Reigosa
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Guangyu ChuLiang Liu
Sep 8, 2019·International Journal of Molecular Sciences·Natalia SiweckaIreneusz Majsterek
Oct 22, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaoqi PanCheng Peng
Jul 23, 2021·Cancer Cell International·Zeeshan JavedJavad Sharifi-Rad

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Methods Mentioned

BETA
ELISA
flow cytometry
electrophoresis

Software Mentioned

- Pad prism
Graph

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