Geno- and cytotoxicity of salinomycin in human nasal mucosa and peripheral blood lymphocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Agmal ScherzadN Kleinsasser

Abstract

Salinomycin is usually applied in stock breading but has also been described as a promising agent against cancer stem cells (CSC). However, knowledge about the toxicity of this ionophor substance is incomplete. The aim of this study was to investigate cyto- and genotoxic effects of salinomycin in human non-malignant cells. Primary human nasal mucosa cells (monolayer and mini organ cultures) and peripheral blood lymphocytes from 10 individuals were used to study the cytotoxic effects of salinomycin (0.1-175 μM) by annexin-propidiumiodide- and MTT-test. The comet assay was performed to evaluate DNA damage. Additionally, the secretion of interleukin-8 was analyzed by ELISA. Flow cytometry and MTT assay revealed significant cytotoxic effects in nasal mucosa cells and lymphocytes at low salinomycin concentrations of 10-20 μM. No genotoxic effects could be observed. IL-8 secretion was elevated at 5 μM. Salinomycin-induced cytotoxic and pro-inflammatory effects were seen at concentrations relevant for anti-cancer treatment. Concurrent to the evaluation of salinomycin application in experimental oncology, adverse effects in non-malignant cells need to be monitored and reduced as much as possible. Further studies are also warranted to e...Continue Reading

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Citations

Nov 19, 2017·Environmental and Molecular Mutagenesis·Helena MoratinStephan Hackenberg
Oct 23, 2015·Cell Death & Disease·A ManagòI Szabò
Aug 4, 2016·The Oncologist·Maximilian BoeschDominik Wolf
Sep 11, 2016·Oncotarget·Gary D R RoulstonAlexander Thompson
Jul 28, 2016·Parasites & Vectors·Dietmar SteverdingAdam Huczyński
Oct 11, 2017·Mutagenesis·Malgorzata M DobrzynskaKatarzyna Owczarska
Mar 22, 2016·Biomedical Reports·Agmal ScherzadNorbert Kleinsasser
Jan 6, 2019·European Journal of Medicinal Chemistry·Michał Antoszczak
Feb 16, 2017·ACS Medicinal Chemistry Letters·David B SmithrudPheruza Tarapore
Nov 23, 2021·Toxicology Mechanisms and Methods·Andressa Megumi NiwaMário Sérgio Mantovani

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