Genome Analysis of Lactobacillus plantarum LL441 and Genetic Characterisation of the Locus for the Lantibiotic Plantaricin C.

Frontiers in Microbiology
Ana B Flórez, Baltasar Mayo

Abstract

Bacteriocins are ribosomally synthesized peptides produced by bacteria with antimicrobial activity. The bacteriocins produced by lactic acid bacteria (LAB) may inhibit food-borne pathogens and spoilage organisms, and therefore have potential as natural preservatives. Lactobacillus plantarum LL441 produces a lantibiotic bacteriocin known as plantaricin C, a pore-forming antimicrobial peptide containing modified amino acids that inhibits cell wall synthesis by forming a complex with the peptidoglycan precursor lipid II. The present work describes the genome sequencing of L. plantarum LL441 and the characterisation of the plantaricin C locus. The draft genome sequence of L. plantarum LL441 consisted of 170 contigs and had a total 3,124,603 bp; the GC content was 44.52%. The plantaricin C locus was found in an 18 kbp-long contig, and consisted of six genes organized in an operon-like arrangement. This locus included the bacteriocin structural gene (plnC), followed by a gene encoding a LanM-like protein thought to be involved in the maturation of plantaricin C, and four downstream genes encoding ABC-type transporter components, probably belonging to its putative immunity and export machinery. plnC encodes a precursor of the bacterio...Continue Reading

References

May 7, 1998·Letters in Applied Microbiology·C M FranzW H Holzapfel
Sep 22, 1999·European Journal of Biochemistry·D L TurnerJ E Suárez
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Apr 11, 2003·Journal of Industrial Microbiology & Biotechnology·Susana Delgado, Baltasar Mayo
Mar 5, 2005·Applied and Environmental Microbiology·Richard van KranenburgMichiel Kleerebezem
Aug 1, 1993·Applied and Environmental Microbiology·D J O'sullivan, T R Klaenhammer
Aug 1, 1996·Applied and Environmental Microbiology·B GonzalezW N Konings
Apr 7, 2006·Applied and Environmental Microbiology·Imke WiedemannBeatriz Martínez
Nov 14, 2006·FEMS Microbiology Reviews·Alain DufourJean-Paul Le Pennec
Jul 7, 2007·International Journal of Food Microbiology·Antonio GálvezNabil Ben Omar
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Mar 20, 2008·Genome Research·Daniel R Zerbino, Ewan Birney
Jun 30, 2009·Applied and Environmental Microbiology·Máire BegleyR Paul Ross
Dec 17, 2009·Environmental Microbiology·Roland J SiezenJohan E T van Hylckama Vlieg
Dec 18, 2009·Nature Chemical Biology·Trent J Oman, Wilfred A van der Donk
Jul 27, 2011·PloS One·Roman K GóreckiJacek K Bardowski
Oct 26, 2011·Microbial Cell Factories·Roland J Siezen, Johan E T van Hylckama Vlieg
Feb 14, 2012·Journal of Bacteriology·Vanessa PittetBarry Ziola
Feb 15, 2012·Journal of Biotechnology·Zainab AlkhatibSander H J Smits
Apr 25, 2012·Applied Microbiology and Biotechnology·Susana LangaJuan M Rodríguez
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qi ZhangWilfred A van der Donk
Nov 12, 2013·International Journal of Medical Microbiology : IJMM·Jasmin DischingerGabriele Bierbaum
Aug 15, 2014·Critical Reviews in Food Science and Nutrition·Vandana BaliJohn F Kennedy
Sep 5, 2014·Microbial Cell Factories·Rodney H PerezKenji Sonomoto
Apr 11, 2015·BioMed Research International·Juan L ArquésMargarita Medina
Jul 21, 2015·Critical Reviews in Food Science and Nutrition·Ramith RamuNagendra Prasad M N
Feb 11, 2016·Applied Microbiology and Biotechnology·Patricia Alvarez-SieiroOscar P Kuipers
Jun 9, 2016·Natural Product Reports·Nadine ZiemertTilmann Weber
Aug 9, 2017·Current Opinion in Biotechnology·Michael L ChikindasLeon Mt Dicks
Oct 17, 2017·Bioinformatics·Luis VielvaFernando de la Cruz
May 23, 2018·Nucleic Acids Research·Auke J van HeelOscar P Kuipers

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA295007

Methods Mentioned

BETA
glycosylation
electrophoresis
PCR
Illumina sequencing

Software Mentioned

Clustal X
Velvet
PLACNET
Velvet assemblage
BLAST
BAGEL
antiSMASH
RAST

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.