Genome and transcriptome analysis of Bacillus velezensis BS-37, an efficient surfactin producer from glycerol, in response to d-/l-leucine

MicrobiologyOpen
Dayuan ZhouShuang Li

Abstract

Surfactin is one of the most widely studied biosurfactants due to its many potential applications in different fields. In the present study, Bacillus velezensis BS-37, initially identified as a strain of Bacillus subtilis, was used to efficiently produce surfactin with the addition of glycerol, an inexpensive by-product of biodiesel production. After 36 hr of growth in glycerol medium, the total surfactin concentration reached more than 1,000 mg/L, which was two times higher than that in sucrose medium. Moreover, the addition of l- and d-Leu to the culture medium had opposite effects on surfactin production by BS-37. While surfactin production increased significantly to nearly 2,000 mg/L with the addition of 10 mM l-Leu, it was dramatically reduced to about 250 mg/L with the addition of 10 mM d-Leu. To systemically elucidate the mechanisms influencing the efficiency of this biosynthesis process, we sequenced the genome of BS-37 and analyzed changes of the transcriptome in glycerol medium in response to d-/l-leucine. The RPKM analysis of the transcriptome of BS-37 showed that the transcription levels of genes encoding modular surfactin synthase, the glycerol utilization pathway, and branched-chain amino acid (BCAA) synthesis pat...Continue Reading

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Citations

Sep 4, 2020·International Journal of Molecular Sciences·Giovanni Andrea VitaleDonatella de Pascale
Jan 30, 2020·Applied Microbiology and Biotechnology·Chenchen ZhangRuixia Gu
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Fazle Rabbee, Kwang-Hyun Baek
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomasz JanekZbigniew Lazar

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Datasets Mentioned

BETA
CP023414

Methods Mentioned

BETA
PCR
DNA assay

Software Mentioned

BLASTP
GraphPad Prism
Adobe Illustrator CC
DESeq
Diamond
Bowtie
GraphPad
prodigal
SMRT Analysis

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