Genome-based classification of Burkholderia cepacia complex provides new insight into its taxonomic status

Biology Direct
Yuan JinJunjie Yue

Abstract

Accurate classification of different Burkholderia cepacia complex (BCC) species is essential for therapy, prognosis assessment and research. The taxonomic status of BCC remains problematic and an improved knowledge about the classification of BCC is in particular needed. We compared phylogenetic trees of BCC based on 16S rRNA, recA, hisA and MLSA (multilocus sequence analysis). Using the available whole genome sequences of BCC, we inferred a species tree based on estimated single-copy orthologous genes and demarcated species of BCC using dDDH/ANI clustering. We showed that 16S rRNA, recA, hisA and MLSA have limited resolutions in the taxonomic study of closely related bacteria such as BCC. Our estimated species tree and dDDH/ANI clustering clearly separated 116 BCC strains into 36 clusters. With the appropriate reclassification of misidentified strains, these clusters corresponded to 22 known species as well as 14 putative novel species. This is the first large-scale and systematic study of the taxonomic status of the BCC and could contribute to further insights into BCC taxonomy. Our study suggested that conjunctive use of core phylogeny based on single-copy orthologous genes, as well as pangenome-based dDDH/ANI clustering wou...Continue Reading

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Citations

May 5, 2021·Current Microbiology·Edgard de Freitas ViannaElizabeth Andrade Marques
Aug 28, 2021·World Journal of Microbiology & Biotechnology·Michelli Inácio Gonçalves FunnicelliEliana Gertrudes de Macedo Lemos
Oct 16, 2021·Frontiers in Microbiology·Alex J Mullins, Eshwar Mahenthiralingam
Nov 4, 2021·Cellular and Molecular Life Sciences : CMLS·Marco FrenschWinfried Römer
Nov 11, 2021·Applied Microbiology and Biotechnology·Viola Camilla ScoffoneSilvia Buroni

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Datasets Mentioned

BETA
PC184

Methods Mentioned

BETA
PCR

Software Mentioned

trimAl
FastANI
FastTree
Muscle
MLSA
iTOL
Phoenix
BLAST
blastn
VITEK

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