Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production

Applied and Environmental Microbiology
Sang Jun LeeSang Yup Lee

Abstract

Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, Mannheimia succiniciproducens, gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation. Among three different CO2-fixing metabolic reactions catalyzed by phosphoenolpyruvate (PEP) carboxykinase, PEP carboxylase, and malic enzyme, PEP carboxykinase was the most important for the anaerobic growth of M. succiniciproducens and succinic acid production. Oxaloacetate formed by carboxylation of PEP was found to be converted to succinic acid by three sequential reactions catalyzed by malate dehydrogenase, fumarase, and fumarate reductase. Major metabolic pathways leading to by-product formation were successfully removed by disrupting the ldhA, pflB, pta, and ackA genes. This metabolically engineered LPK7 strain was able to produce 13.4 g/liter of succinic acid from 20 g/liter glucose with little or no formation of acetic, formic, and lactic acids, resulting in a succ...Continue Reading

References

Aug 26, 1998·Infection and Immunity·J M ReyratR Rappuoli
Jul 6, 2000·Applied and Environmental Microbiology·P GourdonA Guyonvarch
Jun 5, 2003·Archives of Biochemistry and Biophysics·Yasushi KaiKatsura Izui
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Thomas BulterJames C Liao
Mar 17, 2004·Biochimica Et Biophysica Acta·Louis T J DelbaereHughes Goldie
Jun 18, 2004·Bioinformatics·Bo Kyeng HouSang Yup Lee
Sep 17, 2004·Applied Microbiology and Biotechnology·Th Willke, K-D Vorlop
Sep 21, 2004·Nature Biotechnology·Soon Ho HongJae Jong Kim

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Citations

Jul 5, 2007·Applied Microbiology and Biotechnology·James B McKinlayJ Gregory Zeikus
Mar 15, 2008·Applied Microbiology and Biotechnology·Sang Yup LeeYu-Sin Jang
Apr 2, 2008·Applied Microbiology and Biotechnology·Hyohak SongSang Yup Lee
Jun 19, 2012·Applied Microbiology and Biotechnology·Ke-Ke ChengJian-An Zhang
Nov 19, 2009·Bioprocess and Biosystems Engineering·Christian AnderssonUlrika Rova
Feb 26, 2011·Bioprocess and Biosystems Engineering·Tanja Kurzrock, Dirk Weuster-Botz
Mar 14, 2008·Journal of Industrial Microbiology & Biotechnology·Raj KumarOm V Singh
Jun 2, 2009·Journal of Industrial Microbiology & Biotechnology·Shiying LuElliot Altman
Apr 29, 2011·Journal of Industrial Microbiology & Biotechnology·Jing DuHuimin Zhao
Jun 4, 2010·Applied Biochemistry and Biotechnology·Nhuan P NghiemDavid B Johnston
Nov 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xueli ZhangLonnie O Ingram
Jan 2, 2009·The Journal of Biological Chemistry·Alexandra DubiniMatthew C Posewitz
Jun 29, 2010·Applied and Environmental Microbiology·Rintze M ZelleAntonius J A van Maris
Jul 10, 2007·Applied and Environmental Microbiology·Yu-Sin JangKyu Young Kang
Oct 20, 2009·Applied and Environmental Microbiology·X ZhangL O Ingram
Mar 20, 2008·Journal of Molecular Microbiology and Biotechnology·K T Shanmugam, L O Ingram
Oct 14, 2011·Yi chuan = Hereditas·Quan-Feng LiangQing-Sheng Qi
Jun 21, 2014·Microbial Cell Factories·José Manuel Borrero-de AcuñaIgnacio Poblete-Castro
May 22, 2013·BioMed Research International·Ke-Ke ChengJian-An Zhang
Feb 18, 2014·Applied Microbiology and Biotechnology·Svetlana V KamzolovaIgor G Morgunov
Jun 29, 2014·Applied Microbiology and Biotechnology·Svetlana V KamzolovaIgor G Morgunov
May 24, 2011·Nature Chemical Biology·Harry YimStephen Van Dien
May 19, 2012·Nature Chemical Biology·Jeong Wook LeeSang Yup Lee
Nov 21, 2012·Metabolic Engineering·Ignacio Poblete-CastroChristoph Wittmann
Mar 15, 2016·Frontiers in Microbiology·Elizabeth A LathamDavid J Nisbet
Oct 2, 2009·Trends in Biotechnology·Yanping ZhangYin Li
Sep 1, 2015·Current Opinion in Biotechnology·Hannah ChungSang Yup Lee
Sep 2, 2008·Current Opinion in Biotechnology·Jin Hwan Park, Sang Yup Lee
Jun 28, 2008·Trends in Biotechnology·Jin Hwan ParkHyun Uk Kim
Jan 15, 2008·Trends in Biotechnology·Michael SauerPaola Branduardi
Jul 1, 2008·Microbial Biotechnology·Sergio Sanchez, Arnold L Demain
Apr 4, 2007·Biotechnology and Bioengineering·Tae Yong KimSang Yup Lee
Mar 19, 2016·Current Opinion in Biotechnology·Jung Ho AhnSang Yup Lee

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