Genome characterisation of two Ljungan virus isolates from wild bank voles (Myodes glareolus) in Sweden

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Kieran C PounderLorraine M McElhinney

Abstract

Ljungan virus (LV) (family Picornaviridae, genus Parechovirus) is a suspected zoonotic pathogen with associations to human disease in Sweden. LV is a single-stranded RNA virus with a positive sense genome. There are five published Ljungan virus strains, three isolated from Sweden and two from America, and are classified into four genotypes. A further two strains described here were isolated from wild bank voles (Myodes glareolus) caught in Västmanlands county, Sweden in 1994. These strains were sequenced using next generation pyrosequencing technology on the GS454flx platform. Genetic and phylogenetic analysis of the obtained genomes confirms isolates LV340 and LV342 as two new putative members of genotype 2 along with LV145SL, with 92% and 99% nucleotide identities respectively. Only two codon sites throughout the entire genome were identified as undergoing positive selection, both situated within the VP3 structural region, in or near to major antigenic sites. Whilst these two strains do not constitute new genotypes they provide evidence, though weakly supported, which suggests the evolution of Ljungan viruses to be relatively slow, a characteristic unlike other picornaviruses. Additional genomic sequences are urgently require...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T HyypiäG Stanway
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 1, 1990·The Journal of General Virology·G Stanway
Jan 1, 1989·Annual Review of Biochemistry·M G Rossmann, J E Johnson
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S van der WerfJ J Dunn
Nov 20, 1998·The Journal of General Virology·F GhaziG Stanway
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·J W Drake, J J Holland
Feb 1, 2002·Journal of Molecular Evolution·Gareth M JenkinsEdward C Holmes
Aug 8, 2002·Journal of Molecular Evolution·Matthew W DimmicRichard A Goldstein
Mar 15, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M Steven ObersteMark A Pallansch
Mar 26, 2003·The Journal of General Virology·E Susanne JohanssonA Michael Lindberg
May 15, 2003·International Journal of Experimental Diabesity Research·Bo NiklassonAke Lernmark
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Dec 9, 2003·Trends in Microbiology·Edward C Holmes
Feb 11, 2005·Molecular Biology and Evolution·Sergei L Kosakovsky Pond, Simon D W Frost
Feb 17, 2005·Bioinformatics·Sergei L Kosakovsky Pond, Simon D W Frost
Jul 5, 2006·Molecular Biology and Evolution·Sergei L Kosakovsky PondSimon D W Frost
Mar 6, 2007·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Jan 9, 2008·BMC Genomics·Appolinaire DjikengDavid J Spiro
Mar 18, 2008·The Journal of General Virology·K S M BenschopP Simmonds
Aug 5, 2008·Virus Research·Graham J Belsham
Jan 31, 2009·Birth Defects Research. Part A, Clinical and Molecular Teratology·Bo NiklassonWilliam Klitz
Apr 18, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H Harvala, P Simmonds
May 2, 2009·Forensic Science, Medicine, and Pathology·Bo NiklassonWilliam Klitz
Jul 17, 2009·BMC Evolutionary Biology·Nuno R FariaLia van der Hoek
Jul 31, 2010·Bioinformatics·Wayne DelportSergei L Kosakovsky Pond

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Citations

May 22, 2016·The Journal of General Virology·Hiromichi MitakeMakoto Sugiyama
Jun 4, 2020·Vector Borne and Zoonotic Diseases·Cristina FevolaHeidi C Hauffe

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