Genome characterization of the novel lytic Vibrio parahaemolyticus phage vB_VpP_BA6

Archives of Virology
Meiyan YangQingping Wu

Abstract

A lytic bacteriophage, designated Vibrio phage vB_VpP_BA6, was isolated from sewage collected in Guangzhou, China. The double-stranded DNA genome of phage BA6 is composed of 50,520 bp with a G+C content of 41.77%. It possesses 64 open reading frames relating to phage structure, packaging, host lysis, DNA metabolism, and additional functions. Three tRNAs genes (encoding Pro, Ile and Trp) were detected. Comparison of its genomic features and phylogenetic analysis revealed that phage BA6 is a novel member of the family Podoviridae. This phage may represent a potential therapeutic agent against multidrug-resistant Vibrio parahaemolyticus.

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Datasets Mentioned

BETA
MF431726
KJ936628
MK483107

Methods Mentioned

BETA
membrane filter

Software Mentioned

MEGA X
RNAmmer
BLAST
BLASTn
tRNAscan
Prokka
BLASTp
Easyfig
Geneious
SE

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