Genome Complexity Reduction High-Throughput Genome Sequencing of Green Iguana (Iguana iguana ) Reveal a Paradigm Shift in Understanding Sex-Chromosomal Linkages on Homomorphic X and Y Sex Chromosomes

Frontiers in Genetics
Tassika KoomgunKornsorn Srikulnath

Abstract

The majority of lizards classified in the superfamily Iguanoidea have an XX/XY sex-determination system in which sex-chromosomal linkage shows homology with chicken (Gallus gallus) chromosome 15 (GGA15). However, the genomics of sex chromosomes remain largely unexplored owing to the presence of homomorphic sex chromosomes in majority of the species. Recent advances in high-throughput genome complexity reduction sequencing provide an effective approach to the identification of sex-specific loci with both single-nucleotide polymorphisms (SNPs) and restriction fragment presence/absence (PA), and a better understanding of sex chromosome dynamics in Iguanoidea. In this study, we applied Diversity Arrays Technology (DArTseqTM) in 29 phenotypic sex assignments (14 males and 15 females) of green iguana (Iguana iguana). We confirmed a male heterogametic (XX/XY) sex determination mode in this species, identifying 29 perfectly sex-linked SNP/PA loci and 164 moderately sex-linked SNP/PA loci, providing evidence probably indicative of XY recombination. Three loci from among the perfectly sex-linked SNP/PA loci showed partial homology with several amniote sex chromosomal linkages. The results support the hypothesis of an ancestral super-sex ...Continue Reading

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Citations

Nov 5, 2020·Cells·Worapong SingchatKornsorn Srikulnath

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Methods Mentioned

BETA
electrophoresis
PCR
genotyping

Software Mentioned

HapEstXXR
RADseq
R
fastqcoll
DArTseq TM
glPlot
Basic Local Alignment Search Tool ( BLAST )
dartR
DArTseq
R package

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