Jun 18, 2015

Genome Editing in Caenorhabditis briggsae using the CRISPR/Cas9 System

BioRxiv : the Preprint Server for Biology
Elizabeth CulpBhagwati P Gupta

Abstract

The CRISPR/Cas9 system is an efficient technique for generating targeted alterations in an organism's genome. Here we describe a methodology for using the CRISPR/Cas9 system to generate mutations via non-homologous end joining in the nematode Caenorhabditis briggsae, a sister species of C. elegans. Evidence for somatic mutations and off-target mutations are also reported. The use of the CRISPR/Cas9 system in C. briggsae will greatly facilitate comparative studies to C. elegans.

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Mentioned in this Paper

Calcinus elegans
Cyartonema elegans
Coleonyx elegans
Non-Homologous DNA End-Joining
Cestrum elegans
Clarkia unguiculata
Clathrulina elegans
Cardioglossa elegans
Cymbella elegans
Cyrenella elegans

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