Genome Editing in Cereals: Approaches, Applications and Challenges.

International Journal of Molecular Sciences
Waquar A AnsariRupesh Deshmukh

Abstract

Over the past decades, numerous efforts were made towards the improvement of cereal crops mostly employing traditional or molecular breeding approaches. The current scenario made it possible to efficiently explore molecular understanding by targeting different genes to achieve desirable plants. To provide guaranteed food security for the rising world population particularly under vulnerable climatic condition, development of high yielding stress tolerant crops is needed. In this regard, technologies upgradation in the field of genome editing looks promising. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is a rapidly growing genome editing technique being effectively applied in different organisms, that includes both model and crop plants. In recent times CRISPR/Cas9 is being considered as a technology which revolutionized fundamental as well as applied research in plant breeding. Genome editing using CRISPR/Cas9 system has been successfully demonstrated in many cereal crops including rice, wheat, maize, and barley. Availability of whole genome sequence information for number of crops along with the advancement in genome-editing techniques provides several possibilities to achieve desirable traits. In t...Continue Reading

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Citations

Oct 3, 2020·Physiologia Plantarum·M Iqbal R KhanChirag Maheshwari
Jan 30, 2021·Frontiers in Genetics·Ankit VermaPinky Agarwal
Feb 16, 2021·Frontiers in Microbiology·Leena TripathiP Lava Kumar
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Feb 20, 2021·The Application of Clinical Genetics·Nastaran AsriKamran Rostami
Mar 19, 2021·Journal of Agricultural and Food Chemistry·Tian WangHongliang Zhu
Mar 4, 2021·Plant Biotechnology Journal·Wendy J LyzengaSateesh Kagale
May 1, 2021·International Journal of Molecular Sciences·Il-Sup KimWoong-Suk Yang
Apr 10, 2021·Plant Biotechnology Journal·Rim LassouedStuart J Smyth
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Jun 28, 2021·Journal of Biotechnology·Archana SinghIndrakant K Singh
Nov 27, 2021·Molecular Biology Reports·Antony Ceasar

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Methods Mentioned

BETA
gene knock in
transgenic
genome knock in
gene knockout
gene knockouts
transfection
PCR
genetic modifications
GUIDE-seq

Software Mentioned

TALEN
sgRNA
Drosha

Related Concepts

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CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

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