Genome editing in diatoms: achievements and goals

Plant Cell Reports
Peter G KrothAngela Falciatore

Abstract

Diatoms are major components of phytoplankton and play a key role in the ecology of aquatic ecosystems. These algae are of great scientific importance for a wide variety of research areas, ranging from marine ecology and oceanography to biotechnology. During the last 20 years, the availability of genomic information on selected diatom species and a substantial progress in genetic manipulation, strongly contributed to establishing diatoms as molecular model organisms for marine biology research. Recently, tailored TALEN endonucleases and the CRISPR/Cas9 system were utilized in diatoms, allowing targeted genetic modifications and the generation of knockout strains. These approaches are extremely valuable for diatom research because breeding, forward genetic screens by random insertion, and chemical mutagenesis are not applicable to the available model species Phaeodactylum tricornutum and Thalassiosira pseudonana, which do not cross sexually in the lab. Here, we provide an overview of the genetic toolbox that is currently available for performing stable genetic modifications in diatoms. We also discuss novel challenges that need to be addressed to fully exploit the potential of these technologies for the characterization of diato...Continue Reading

References

Oct 16, 1996·Molecular & General Genetics : MGG·K E AptA R Grossman
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BitinaiteI Schildkraut
Jun 29, 1999·Marine Biotechnology·A FalciatoreC Bowler
Oct 3, 2002·Journal of Cell Science·Kirk E AptPeter G Kroth
Nov 30, 2006·Nucleic Acids Research·Julianne SmithPhilippe Duchateau
Oct 2, 2007·Current Opinion in Biotechnology·Dagmar WirthHansjörg Hauser
Feb 20, 2008·Journal of Biotechnology·Gali Harel-LeviMikhail Kolot
Nov 6, 2008·PloS One·Carola EnglerSylvestre Marillonnet
May 16, 2009·Nature·E Virginia Armbrust
Jun 3, 2009·Nucleic Acids Research·Valentina De RisoAngela Falciatore
Nov 26, 2009·Science·Matthew J Moscou, Adam J Bogdanove
Jul 28, 2010·Genetics·Michelle ChristianDaniel F Voytas
Mar 3, 2011·PloS One·Ernst WeberSylvestre Marillonnet
Oct 19, 2011·Microbial Cell Factories·Franziska HempelUwe G Maier
Jan 10, 2012·Nature Protocols·Neville E SanjanaFeng Zhang
Jun 8, 2012·Biotechnology for Biofuels·Jacob ValenzuelaMatthew W Fields
Jan 5, 2013·Science·Le CongFeng Zhang
Apr 9, 2013·Bioscience, Biotechnology, and Biochemistry·Mado MiyaharaKentaro Ifuku
Apr 25, 2013·Nature Communications·Marine BeurdeleyGeorge H Silva
Dec 18, 2013·Science·Ophir ShalemFeng Zhang
Jan 21, 2014·Applied Microbiology and Biotechnology·Nafiseh Nafissi, Roderick Slavcev
Jan 21, 2014·Nucleic Acids Research·Yanni LinGang Bao
Mar 5, 2014·Biochemical and Biophysical Research Communications·Yong WuDesheng Liang
May 30, 2014·Nature Communications·Fayza DaboussiPhilippe Duchateau
Oct 10, 2014·Photosynthesis Research·Kentaro IfukuYasuhiro Kashino
Nov 7, 2014·PloS One·Francisco Javier Buitrago-FlórezDiego Mauricio Riaño-Pachón
Nov 29, 2014·Science·Jennifer A Doudna, Emmanuelle Charpentier
Dec 23, 2014·Scientific Reports·Xiangyang ChenShouhong Guang
Apr 22, 2015·Nature Communications·Bogumil J KarasPhilip D Weyman
Jul 15, 2015·Journal of Experimental Botany·Leila AlipanahTore Brembu
Oct 7, 2015·Current Biology : CB·John M Archibald

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Citations

May 10, 2019·Marine Drugs·Chiara LauritanoAlessandra Rogato
Apr 11, 2020·Physiologia Plantarum·Eva M Farré
Sep 10, 2018·Plant Cell Reports·C Neal StewartJoseph M Jez
Dec 12, 2019·Frontiers in Plant Science·Eric Maréchal
Jul 22, 2019·Biotechnology Advances·Anastasia Athanasakoglou, Sotirios C Kampranis
Jan 20, 2021·Scientific Reports·Rossella AnnunziataMaria Immacolata Ferrante
Jan 27, 2021·International Journal of Molecular Sciences·Eli BonneureSven Mangelinckx
Jan 26, 2021·Bioresource Technology·Raya BhattacharjyaShireesh Srivastava
Oct 19, 2021·Frontiers in Bioengineering and Biotechnology·Gilles DefrelFayza Daboussi

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Methods Mentioned

BETA
transgenic
PCR
gene knockout

Software Mentioned

cTALEN
Modular Cloning ( MoClo )
cTALENs

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