Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles

Methods in Molecular Biology
Carsten PohlYvonne Nygård

Abstract

Several CRISPR/Cas9 tools have been recently established for precise genome editing in a wide range of filamentous fungi. This genome editing platform offers high flexibility in target selection and the possibility of introducing genetic deletions without the introduction of transgenic sequences . This chapter describes an approach for the transformation of Penicillium chrysogenum protoplasts with preassembled ribonucleoprotein particles (RNPs) consisting of purified Cas9 protein and in vitro transcribed single guide RNA (sgRNA) for the deletion of genome sequences or their replacement with alternative sequences. This method is potentially transferable to all fungal strains where protoplasts can be obtained from.

Citations

May 16, 2019·FEMS Microbiology Letters·Belén Adiego-PérezJohn van der Oost
Nov 20, 2019·Microbial Cell Factories·László MózsikYvonne Nygård
Dec 14, 2018·Frontiers in Microbiology·Fernando Guzmán-ChávezArnold J M Driessen
Mar 5, 2019·Frontiers in Plant Science·Isabel Vicente MuñozGiovanni Vannacci
Jan 2, 2019·Fungal biology and biotechnology·Alexander Idnurm, Vera Meyer
Jan 15, 2021·Scientific Reports·László MózsikArnold J M Driessen

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