Genome editing methods in animal models.

Animal Cells and Systems
Hyunji LeeKyoungmi Kim

Abstract

Genetically engineered animal models that reproduce human diseases are very important for the pathological study of various conditions. The development of the clustered regularly interspaced short palindromic repeats (CRISPR) system has enabled a faster and cheaper production of animal models compared with traditional gene-targeting methods using embryonic stem cells. Genome editing tools based on the CRISPR-Cas9 system are a breakthrough technology that allows the precise introduction of mutations at the target DNA sequences. In particular, this accelerated the creation of animal models, and greatly contributed to the research that utilized them. In this review, we introduce various strategies based on the CRISPR-Cas9 system for building animal models of human diseases and describe various in vivo delivery methods of CRISPR-Cas9 that are applied to disease models for therapeutic purposes. In addition, we summarize the currently available animal models of human diseases that were generated using the CRISPR-Cas9 system and discuss future directions.

References

Apr 28, 2007·Biochemical and Biophysical Research Communications·GuanFeng YaoKangMei Kong
Feb 18, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nicole K PaulkMarkus Grompe
Jan 5, 2013·Science·Le CongFeng Zhang
Apr 3, 2013·Cell Research·Bin ShenXingxu Huang
Apr 1, 2014·Nature Biotechnology·Hao YinDaniel G Anderson
Sep 30, 2014·Cell·Randall J PlattFeng Zhang
Oct 3, 2014·Cellular and Molecular Life Sciences : CMLS·Xiaoqing ZhouLiangxue Lai
Apr 2, 2015·Nature·F Ann RanFeng Zhang
Apr 11, 2015·Human Molecular Genetics·Yongchang ChenXiao-Jiang Li
Oct 16, 2015·Journal of Molecular Cell Biology·Qingjian ZouXiang Gao
Dec 17, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Benjamin BakondiShaomei Wang
Jan 2, 2016·Science·Mohammadsharif TabebordbarAmy J Wagers
Apr 27, 2016·Scientific Reports·Qingyan LvLiangxue Lai
Jan 1, 2015·Nature Reviews. Disease Primers·Gillian P BatesSarah J Tabrizi
Nov 11, 2016·Nature·Tony Wyss-Coray
Jan 28, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alex Mas MonteysBeverly L Davidson
Feb 22, 2017·Nature Communications·Eunji KimJin-Soo Kim
Mar 1, 2017·Nature Biotechnology·Kyoungmi KimJin-Soo Kim
Apr 4, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chaoran YinWenhui Hu
Jul 26, 2017·Nature Communications·Xionggao HuangHetian Lei
Dec 10, 2017·Nature Communications·Miguel A Moreno-MateosAntonio J Giraldez
Jun 7, 2018·Disease Models & Mechanisms·Tingting SuiRenzhi Han
Jun 29, 2018·G3 : Genes - Genomes - Genetics·Yuanyuan XuZhanjun Li

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Citations

Jun 9, 2020·Clinical Genetics·Roya RamezankhaniCatherine M Verfaillie
Dec 17, 2020·Expert Opinion on Drug Discovery·Laure Grand MourselAnne-Marie Zuurmond
Jan 1, 2021·Expert Opinion on Drug Discovery·Kenneth P SeastedtChuong D Hoang
Dec 3, 2020·Animals : an Open Access Journal From MDPI·Md Aminul IslamHaruki Kitazawa
Dec 16, 2020·BMC Biology·Nicholas BrookhouserDavid A Brafman
Feb 19, 2021·Laboratory Animals·Federico Caso, Benjamin Davies
Feb 4, 2021·Aging and Disease·Minjing KeHuanxing Su
Aug 18, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Takafumi NamikiNaomi Kashiwazaki

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Methods Mentioned

BETA
gene knockin
transgenic

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