Genome editing of Francisella tularensis using (CRISPR-Cas9).

Journal of Microbiological Methods
Stephanie J Southern, Petra C F Oyston

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system is a powerful tool for gene editing in eukaryotic genomes but is still being developed for editing bacterial genomes. Here we describe the construction of an all-in-one vector for generating potentially scarless deletion mutants in Francisella tularensis LVS using a CRISPR-Cas9-based system.

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