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Genome engineering using the CRISPR-Cas9 system

Nature Protocols

Oct 26, 2013

F Ann RanFeng Zhang

Abstract

Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in ...read more

Mentioned in this Paper

Non-Homologous DNA End-Joining
Immune System
Transfection
HEK293 Cells
Genome
Graft-vs-Host Disease
Base Excision Repair
Cell Culture Techniques
Cytokinesis of the Fertilized Ovum
Alkaline DNase
43
10
8
10K+
6
Paper Details
References
  • References64
  • Citations1666
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  • References64
  • Citations1666
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Genome engineering using the CRISPR-Cas9 system

Nature Protocols

Oct 26, 2013

F Ann RanFeng Zhang

PMID: 24157548

DOI: 10.1038/nprot.2013.143

Abstract

Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in ...read more

Mentioned in this Paper

Non-Homologous DNA End-Joining
Immune System
Transfection
HEK293 Cells
Genome
Graft-vs-Host Disease
Base Excision Repair
Cell Culture Techniques
Cytokinesis of the Fertilized Ovum
Alkaline DNase
43
10
8
10K+
6

Feeds With Similar Papers

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Related Papers

Science

Multiplex genome engineering using CRISPR/Cas systems

ScienceJanuary 5, 2013
Le CongFeng Zhang
Paper Details
References
  • References64
  • Citations1666
12345...
  • References64
  • Citations1666
12345...

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