PMID: 8582347Sep 1, 1995Paper

Genome fingerprinting of the silkworm, Bombyx mori, using random arbitrary primers

Electrophoresis
G M Nagaraja, J Nagaraju

Abstract

The random amplified polymorphic DNA (RAPD) technique was used to study DNA profiling of thirteen silkworm genotypes. The genotypes included six diapausing and seven nondiapausing varieties that represent a high degree of divergence with respect to geographic origin, and morphological, qualitative, quantitative and biochemical characters. Two hundred sixteen amplified products were generated using 40 random primers. Genotype-specific amplification products were identified. Amplification products specific to diapausing genotypes were also identified. Segregation of the RAPD marker was analyzed in a backcross population and found to be inherited as dominant Mendelian traits. Based on pairwise comparison of amplified products, the genetic similarity was performed by a hierarchical clustering technique. Silkworm genotypes were clustered into two groups, one consisting of six diapausing and the other of seven nondiapausing genotypes. The results of our study suggest that the RAPD technique could be used as a powerful tool to generate genetic markers that are linked to traits of interest in the silkworm.

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Citations

Oct 18, 2002·Molecular Phylogenetics and Evolution·M Dharma PrasadJavaregowda Nagaraju
Jun 19, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Min-Jin HanZe Zhang
Jan 8, 2011·Molecular Biology and Evolution·Hong-Song YuZe Zhang
Feb 5, 2000·Genome Génome / Conseil National De Recherches Canada·K D ReddyJ Nagaraju
May 15, 2008·Bioinformation·Nicole KoshyS M H Qadri
Jun 27, 2014·Genome Biology and Evolution·Min-Jin HanZe Zhang
Sep 9, 2010·Annual Review of Entomology·Suresh Kumar RainaSusie Wren
Jan 22, 2003·Insect Biochemistry and Molecular Biology·P PriyadarshiniL Singh
Jun 30, 2012·Science China. Life Sciences·Wei SunZe Zhang
Oct 20, 2004·Journal of Invertebrate Pathology·A M KhuradJ Nagaraju
May 23, 2006·Journal of Invertebrate Pathology·Arun M KhuradJavaregowda Nagaraju
Aug 21, 2013·Insect Science·Sasibhushan SirigineediKangayam M Ponnuvel
Oct 6, 2017·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Muhammad AkmalMuhammad Razaq
Sep 5, 2006·Molecular and Cellular Biochemistry·Ranbir Chander SobtiSavita Bisnoi
Jul 2, 2018·Genes & Genomics·Min-Jin HanHua-Hao Zhang
Oct 13, 2020·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Chunlin LiFangyin Dai

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