Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization-Biotechnological Potential and Biosafety Considerations

International Journal of Environmental Research and Public Health
Krzysztof PoszytekLukasz Dziewit

Abstract

Sewage sludge is an abundant source of microorganisms that are metabolically active against numerous contaminants, and thus possibly useful in environmental biotechnologies. However, amongst the sewage sludge isolates, pathogenic bacteria can potentially be found, and such isolates should therefore be carefully tested before their application. A novel bacterial strain, Ochrobactrum sp. POC9, was isolated from a sewage sludge sample collected from a wastewater treatment plant. The strain exhibited lipolytic, proteolytic, cellulolytic, and amylolytic activities, which supports its application in biodegradation of complex organic compounds. We demonstrated that bioaugmentation with this strain substantially improved the overall biogas production and methane content during anaerobic digestion of sewage sludge. The POC9 genome content analysis provided a deeper insight into the biotechnological potential of this bacterium and revealed that it is a metalotolerant and a biofilm-producing strain capable of utilizing various toxic compounds. The strain is resistant to rifampicin, chloramphenicol and β-lactams. The corresponding antibiotic resistance genes (including blaOCH and cmlA/floR) were identified in the POC9 genome. Nevertheless,...Continue Reading

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Citations

Jul 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krzysztof PoszytekLukasz Drewniak
Mar 22, 2020·International Journal of Molecular Sciences·Przemyslaw DecewiczLukasz Dziewit
May 6, 2019·International Journal of Environmental Research and Public Health·Amanda PacholakEwa Kaczorek
Aug 15, 2018·International Journal of Environmental Research and Public Health·Magdalena LebiockaAgnieszka Cydzik-Kwiatkowska
Oct 31, 2019·Genes·Krzysztof RomaniukLukasz Dziewit
Nov 2, 2020·Folia Microbiologica·Biplab DashRavindra Soni

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Methods Mentioned

BETA
PCR

Software Mentioned

RPSBLAST
Magellan
RPS
CutAdapt
EUCAST
SE
KEGG Automatic Annotation System ( KAAS )
PATRIC
BLAST
RAST

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