Feb 14, 2014

Genome-guided transcriptome assembly in the age of next-generation sequencing

IEEE/ACM Transactions on Computational Biology and Bioinformatics
Liliana Florea, Steven L Salzberg

Abstract

Next-generation sequencing technologies provide unprecedented power to explore the repertoire of genes and their alternative splice variants, collectively defining the transcriptome of a species in great detail. However, assembling the short reads into full-length gene and transcript models presents significant computational challenges. We review current algorithms for assembling transcripts and genes from next-generation sequencing reads aligned to a reference genome, and lay out areas for future improvements.

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Mentioned in this Paper

In Silico
Genome
Genes
Sequence Determinations, RNA
Splice Variants, Protein
Genome Mapping
Massively-Parallel Sequencing
Species
Transcriptome
Gene Expression Profiles

About this Paper

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