Genome mining for natural product biosynthetic gene clusters in the Subsection V cyanobacteria

BMC Genomics
Melinda L MicallefMichelle C Moffitt

Abstract

Cyanobacteria are well known for the production of a range of secondary metabolites. Whilst recent genome sequencing projects has led to an increase in the number of publically available cyanobacterial genomes, the secondary metabolite potential of many of these organisms remains elusive. Our study focused on the 11 publically available Subsection V cyanobacterial genomes, together with the draft genomes of Westiella intricata UH strain HT-29-1 and Hapalosiphon welwitschii UH strain IC-52-3, for their genetic potential to produce secondary metabolites. The Subsection V cyanobacterial genomes analysed in this study are reported to produce a diverse range of natural products, including the hapalindole-family of compounds, microcystin, hapalosin, mycosporine-like amino acids and hydrocarbons. A putative gene cluster for the cyclic depsipeptide hapalosin, known to reverse P-glycoprotein multiple drug resistance, was identified within three Subsection V cyanobacterial genomes, including the producing cyanobacterium H. welwitschii UH strain IC-52-3. A number of orphan NRPS/PKS gene clusters and ribosomally-synthesised and post translationally-modified peptide gene clusters (including cyanobactin, microviridin and bacteriocin gene clu...Continue Reading

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Jun 9, 2016·Natural Product Reports·Nadine ZiemertTilmann Weber
Dec 7, 2018·Applied and Environmental Microbiology·Jan MarešPavel Hrouzek
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Datasets Mentioned

BETA
KF742386
KF742388
CAQ16121
CAQ16116
BAG02233
ACC54551-ACC54552
BC008
000472885.1

Methods Mentioned

BETA
Illumina sequencing
PCR
sulfotransferase

Software Mentioned

BLASTp
antiSMASH
NRPSpredictor2
Geneious
BLASTp ( Basic Local Alignment Search Tool )
COG
Glimmer
NaPDoS
Clustal Omega
Biomatters

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