Genome mining for peptidases in heat-tolerant and mesophilic fungi and putative adaptations for thermostability

BMC Genomics
Tássio Brito de OliveiraAndre Rodrigues

Abstract

Peptidases (EC 3.4) consist of a large group of hydrolytic enzymes that catalyze the hydrolysis of proteins accounting for approximately 65% of the total worldwide enzyme production. Peptidases from thermophilic fungi have adaptations to high temperature that makes them adequate for biotechnological application. In the present study, we profiled the genomes of heat-tolerant fungi and phylogenetically related mesophilic species for genes encoding for peptidases and their putative adaptations for thermostability. We generated an extensive catalogue of these enzymes ranging from 241 to 820 peptidase genes in the genomes of 23 fungi. Thermophilic species presented the smallest number of peptidases encoding genes in relation to mesophilic species, and the peptidases families with a greater number of genes were the most affected. We observed differences in peptidases in thermophilic species in comparison to mesophilic counterparts, at (i) the genome level: a great reduction in the number of peptidases encoding genes that harbored a higher number of copies; (ii) in the primary protein structure: shifts in proportion of single or groups of amino acids; and (iii) in the three-dimensional structure: reduction in the number of internal ca...Continue Reading

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Datasets Mentioned

BETA
JSYX01.1

Software Mentioned

Blast
JVirGel
Augustus
PROCHECK
PdbViewer
PhyML
GeneMark
BioNJ
SIMPER
ProSa

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