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Genome mining in Streptomyces clavuligerus: expression and biochemical characterization of two new cryptic sesquiterpene synthases

Chemistry & Biology

Feb 1, 2011

Yunfeng HuDavid E Cane

Abstract

Two presumptive terpene synthases of unknown biochemical function encoded by the sscg_02150 and sscg_03688 genes of Streptomyces clavuligerus ATCC 27074 were individually expressed in Escherichia coli as N-terminal-His₆-tag proteins, using codon-optimized synthetic genes. Incubation of ...read more

Mentioned in this Paper

Metabolic Process, Cellular
Molecular Stereochemistry
farnesyl pyrophosphate, (Z,Z)-isomer
Biochemical Pathway
Alcohols
Transverse Tubule of Muscle Cell
Scapania undulata
Anemone americana
ATTS1 protein, Arabidopsis
CFC1 gene
55
2
Paper Details
References
  • References14
  • Citations33
12
  • References14
  • Citations33
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Genome mining in Streptomyces clavuligerus: expression and biochemical characterization of two new cryptic sesquiterpene synthases

Chemistry & Biology

Feb 1, 2011

Yunfeng HuDavid E Cane

PMID: 21276937

DOI: 10.1016/j.chembiol.2010.11.008

Abstract

Two presumptive terpene synthases of unknown biochemical function encoded by the sscg_02150 and sscg_03688 genes of Streptomyces clavuligerus ATCC 27074 were individually expressed in Escherichia coli as N-terminal-His₆-tag proteins, using codon-optimized synthetic genes. Incubation of ...read more

Mentioned in this Paper

Metabolic Process, Cellular
Molecular Stereochemistry
farnesyl pyrophosphate, (Z,Z)-isomer
Biochemical Pathway
Alcohols
Transverse Tubule of Muscle Cell
Scapania undulata
Anemone americana
ATTS1 protein, Arabidopsis
CFC1 gene
55
2

Feeds With Similar Papers

Proteogenomics

Proteogenomics is a multi-omics research field that has the aim to efficiently integrate genomics, transcriptomics and proteomics. Here is the latest research on proteogenomics.

Structure and Function of DNA

The double helix structure of DNA is important for its biological function. Discover the latest research on the Structure and Function of DNA here.

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Paper Details
References
  • References14
  • Citations33
12
  • References14
  • Citations33
1234

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