Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins

Genes
Katrin ViigandTiina Alamäe

Abstract

Genomic clustering of functionally related genes is rare in yeasts and other eukaryotes with only few examples available. Here, we summarize our data on a nontelomeric MAL cluster of a non-conventional methylotrophic yeast Ogataea (Hansenula) polymorpha containing genes for α-glucosidase MAL1, α-glucoside permease MAL2 and two hypothetical transcriptional activators. Using genome mining, we detected MAL clusters of varied number, position and composition in many other maltose-assimilating non-conventional yeasts from different phylogenetic groups. The highest number of MAL clusters was detected in Lipomyces starkeyi while no MAL clusters were found in Schizosaccharomyces pombe and Blastobotrys adeninivorans. Phylograms of α-glucosidases and α-glucoside transporters of yeasts agreed with phylogenesis of the respective yeast species. Substrate specificity of unstudied α-glucosidases was predicted from protein sequence analysis. Specific activities of Scheffersomycesstipitis α-glucosidases MAL7, MAL8, and MAL9 heterologously expressed in Escherichia coli confirmed the correctness of the prediction-these proteins were verified promiscuous maltase-isomaltases. α-Glucosidases of earlier diverged yeasts L. starkeyi, B. adeninivorans a...Continue Reading

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Citations

Jan 8, 2020·International Journal of Molecular Sciences·Triinu VisnapuuTiina Alamäe
Nov 4, 2020·Yeast·Anjan VenkateshKenneth H Wolfe

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Methods Mentioned

BETA
Assay
Protein Assay

Software Mentioned

ClustalW
MycoCosm
Tecan i - control
pDRAW32
MEGA
Blast

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