Genome nucleotide lengths that are divisible by six are not essential but enhance replication of defective interfering RNAs of the paramyxovirus simian virus 5

Virology
S K Murphy, G D Parks

Abstract

For some members of the Paramyxoviridae family of negative strand RNA viruses, efficient genome replication only occurs when the total genome length is a multiple of six (6N length, where N is any integer). To determine if this "rule of six" requirement applied to the replication of the prototype paramyxovirus simian virus 5 (SV5), defective interfering (DI) RNA genomes were generated by sequential undiluted passage of virus in tissue culture. Molecular cloning and nucleotide sequence analysis of 10 RNA genomes revealed a series of copyback DI RNAs with chain lengths between 449 and 1365 bases, but only 4 of the 10 naturally occurring RNA genomes were of 6N length. Many of the cloned DI genomes could be grouped into two distinct nested sets, with the members of each set having the same polymerase crossover junctions and extent of terminal complementarity but differing from each other by internal deletions. One of these nested sets of genomes consisted of novel DI RNAs that contained a pentameric stretch of nontemplated adenosine residues inserted precisely at the polymerase crossover junction. A reverse genetics system was established in which SV5 DI genomes were replicated in vivo entirely by cDNA-derived components. Using thi...Continue Reading

References

Jan 1, 1976·Virology·R A LambP W Choppin
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K H ParkM Krystal
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P L CollinsD S Stec
May 1, 1991·Genes & Development·J P Jacques, D Kolakofsky
May 1, 1989·Journal of Virology·E H EgelmanG Murti
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Feb 1, 1994·Journal of Virology·K K Conzelmann, M Schnell

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Citations

May 15, 2002·The EMBO Journal·Monika RagerRoberto Cattaneo
Sep 3, 2004·Nucleic Acids Research·Jennifer T MillerStuart F J Le Grice
May 6, 2011·Journal of Virology·Christian J SauderSteven A Rubin
Apr 25, 2000·Journal of Virology·D K ClarkeS A Udem
Apr 20, 2001·Journal of Virology·D Vulliémoz, L Roux
Jun 20, 2001·Journal of Virology·V von MesslingR Cattaneo
Apr 5, 2002·Journal of Virology·Veronika von Messling, Roberto Cattaneo
Jul 19, 2002·The Journal of General Virology·David BhellaRobert P Yeo
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Mar 13, 2001·Methods : a Companion to Methods in Enzymology·C KaoM Zheng
Feb 21, 2002·Virology·Griffith D ParksKelly M Cooke
Jan 5, 2005·Virology·Fernando MarcosEnrique Villar
Jun 1, 2009·Viruses·Carmen YeaRaymond Tellier
Sep 19, 2003·The Journal of General Virology·Elizabeth R ChareEdward C Holmes
Feb 26, 2020·Pathogens·Daniela Ogonczyk MakowskaGuy Boivin
Mar 21, 2017·Archives of Virology·Aidin Molouki, Ben Peeters

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